Phase Separation Multi-phase Flow Using an Aqueous Two-phase System of a Polyethylene Glycol/Dextran Mixed Solution
Phase Separation Multi-phase Flow Using an Aqueous Two-phase System of a Polyethylene Glycol/Dextran Mixed Solution
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DOI：
10.2116/analsci.18p105
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发表时间：
2018-08-01
影响因子：
1.6
通讯作者：
Murata, Masaharu
中科院分区：
文献类型：
--
作者：
Imanishi, Naoya;Yamasaki, Tetsuo;Murata, Masaharu
聚乙二醇/葡聚糖混合溶液作为双水相体系，在不同条件下进入熔融石英毛细管，发生相变，导致相分离多相流通过/沿液-液界面流。作为流型的一个例子，当6.4wt%的聚乙二醇水溶液和9.7wt%的含有1.0 mm罗丹明B的葡聚糖水溶液在3℃下加入毛细管中时，通过明场显微镜观察到管径向分布流动(年流量)。管径向分布流由富含葡聚糖的内相和富含聚乙二醇的外相组成。我们还通过使用不同内径的双毛细管，检测了蛋白质如牛血清白蛋白、血红蛋白和溶菌酶在内相和外相中的分布。内相(富含葡聚糖)的蛋白质分布大于外相(富含聚乙二醇相)。三种蛋白质的分布比(内/外蛋白质浓度之比)分别为2.3、4.2和1.8。在聚乙二醇/葡聚糖混合溶液的管径向分布流中，蛋白质集中在富葡聚糖相。
Polyethylene glycol/dextran mixed solution as an aqueous two-phase system was fed into a fused-silica capillary tube under different conditions, resulting in phase transformation leading to phase separation multi-phase flow through/along a liquid-liquid interface. As one flow-type example, when 6.4 wt% polyethylene glycol and 9.7 wt% dextran aqueous solution containing 1.0 mM Rhodamine B was fed into the capillary tube at 3 degrees C, tube radial distribution flow (annual flow) was observed through bright-field microscopy. Tube radial distribution flow consisted of a dextran-rich inner phase and polyethylene glycol-rich outer phase. We also examined the distribution of proteins, such as bovine serum albumin, hemoglobin, and lysozyme, in the inner and outer phases through use of double capillary tubes with different inner diameters. The protein distribution was greater in the inner (dextran-rich) phase than the outer (polyethylene glycol-rich) phase. The distribution ratios of the three proteins (ratio of the inner/outer protein concentration) were 2.3, 4.2, and 1.8, respectively. The proteins concentrated in the dextran-rich phase through tube radial distribution flow of a polyethylene glycol/dextran mixed solution.