F-19 NMR-STUDY OF 5-FLUOROTRYPTOPHAN-LABELED BACTERIORHODOPSIN
F-19 NMR-STUDY OF 5-FLUOROTRYPTOPHAN-LABELED BACTERIORHODOPSIN
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DOI：
10.1016/0014-5793(87)81506-5
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发表时间：
1987-03-23
期刊：
影响因子：
3.5
通讯作者：
OVCHINNIKOV, YA
中科院分区：
文献类型：
--
作者：
ARSENIEV, AS;KURYATOV, AB;OVCHINNIKOV, YA
核磁共振和CD光谱显示，在CH/sub - 3/OH-CHCl/sub - 3/混合物中溶解的细菌视紫红质及其5-荧光色氨酸标记的类似物(i)保留了紫色膜中完全活性的色蛋白的二级结构，（ii）具有折叠结构，其中Lys-216结合的视网膜上的修饰被顺序远端色氨酸残基感知。经过有限的蛋白水解和细菌视紫红质的NaBH/sub - 4/-裂解后分离的单个片段在有机溶剂中保持了完整多肽链的空间结构。16。参考文献;7无花果。
/sup 19/F NMR and CD spectra reveal that bacteriorhodopsin as well as its 5-fluorotryptophan-labeled analog solubilized in a CH/sub 3/OH-CHCl/sub 3/ mixture (i) retains a secondary structure of the fully active chromoprotein in the purple membrane and (ii) possesses a folded structure in which modifications at the Lys-216 bound retinal are sensed by sequentially remote tryptophan residues. Individual fragments isolated after limited proteolysis and NaBH/sub 4/-cleavage of bacteriorhodopsin keep the spatial structure of the intact polypeptide chain in the organic solvent. 16 refs.; 7 figs.