Expressions of putative cancer stem cell markers ABCB1, ABCG2, and CD133 are correlated with the degree of differentiation of gastric cancer
Expressions of putative cancer stem cell markers ABCB1, ABCG2, and CD133 are correlated with the degree of differentiation of gastric cancer
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DOI：
10.1007/s10120-012-0140-y
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发表时间：
2012-10-01
期刊：
影响因子：
7.4
通讯作者：
Jin, Xiao-Ming
中科院分区：
文献类型：
--
作者：
Jiang, Yang;He, Yan;Jin, Xiao-Ming
本研究旨在确定3种肿瘤干细胞（cancer stem cell， CSC）标志物的表达与胃癌分化程度之间是否存在定量关系。用免疫荧光法检测90例人胃腺癌组织中3种可能的CSC标志物ABCB1、ABCG2和CD133的表达。采用3-[4,5-二甲基噻唑-2基]-2,5-二苯基溴化四唑（MTT）法，观察并比较3种胃癌细胞株（未分化胃癌细胞株HGC-27、低分化胃癌细胞株BGC-823和中低分化胃癌细胞株SGC-7901）的分化情况。构建裸鼠异种移植胃癌，比较3种不同分化的胃癌细胞系的恶性程度。体外流式细胞术检测3种胃癌细胞系中3种推定的CSC标记物的表达，免疫荧光染色法检测3种胃癌异种移植肿瘤细胞中3种推定的CSC标记物的表达。ABCB1、ABCG2和CD133的表达普遍与胃癌的分化程度相关。在人胃腺癌和胃癌细胞系中，ABCB1、ABCG2和CD133的表达随着胃癌恶性程度的增加而增加。在人胃腺癌中，低分化腺癌比高分化腺癌表达更多的ABCB1、ABCG2和CD133。此外，ABCB1和CD133在弥漫型胃癌中的表达高于肠型胃癌。未分化细胞系HGC-27比中等低分化细胞系SGC-7901表达更多的假定的CSC标记。在3种胃癌细胞系产生的异种移植肿瘤中也观察到类似的结果。CSC标志物ABCB1、ABCG2、CD133在不同分化程度的胃癌中表达不同，低分化胃癌中CSC标志物表达相对较多。
The present study was carried out to determine whether a quantitative relationship exists between the expressions of 3 cancer stem cell (CSC) markers and the degree of differentiation of gastric cancer.The expressions of 3 putative CSC markers, ABCB1, ABCG2, and CD133, were detected in 90 human gastric adenocarcinoma cases by immunofluorescence assay. The differentiation statuses of 3 gastric cancer cell lines (the undifferentiated gastric cancer cell line HGC-27, the poorly differentiated gastric cancer cell line BGC-823, and the moderately-poorly differentiated gastric adenocarcinoma cell line SGC-7901) were observed and compared by performing the 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyl tetrazolium bromide (MTT) assay. Gastric xenotransplant cancers in nude mice were constructed to compare the malignancy of the 3 variously differentiated gastric cancer cell lines. The expressions of the 3 putative CSC markers were also detected in the 3 gastric cancer cell lines in vitro by flow cytometric analysis and in the 3 gastric xenotransplant cancers in vivo by immunofluorescence staining.The expressions of ABCB1, ABCG2, and CD133 were generally correlated with the degree of differentiation of the gastric cancers. In the human gastric adenocarcinomas and in the cancer cell lines, the expressions of ABCB1, ABCG2, and CD133 increased with the increases in the malignancy grades of the gastric cancers. In the human gastric adenocarcinomas, poorly differentiated adenocarcinoma expressed more ABCB1, ABCG2, and CD133 than well-differentiated adenocarcinoma. In addition, the expressions of ABCB1 and CD133 were higher in the diffuse type than in the intestinal type of human gastric cancers. The undifferentiated cell line HGC-27 expressed more putative CSC markers than the moderately-poorly differentiated cell line SGC-7901. Similar results were observed in the xenotransplant tumors that arose from the 3 gastric cancer cell lines.The expressions of the CSC markers ABCB1, ABCG2, and CD133 differed in the gastric cancers with various degrees of differentiation, with poorly differentiated gastric cancer expressing relatively more CSC markers.