Candidate proteins involved in the calyx abscission process of 'Kuerlexiangli' (Pyrus sinkiangensisYu) identified by iTRAQ analysis
Candidate proteins involved in the calyx abscission process of 'Kuerlexiangli' (Pyrus sinkiangensisYu) identified by iTRAQ analysis
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通过 iTRAQ 分析鉴定出参与“库尔勒相里”(PyrusinkiangensisYu)花萼脱落过程的候选蛋白
DOI：
10.1007/s11738-020-03097-x
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发表时间：
2020-06-12
影响因子：
2.6
通讯作者：
Tao, Shutian
中科院分区：
文献类型：
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作者：
Gong, Xin;Bao, Jianping;Tao, Shutian
库尔勒香梨是我国重要的商品梨。果的形状和质量受到宿存花萼的存在的负面影响。为了探讨花萼脱落的分子机制，在三种处理下，在花萼脱落的两个关键过程中鉴定了不同的蛋白质表达：一种处理（6000 x氟硅唑+300 x PBO），一种持续处理（50 mg/L GA（3））和一种水对照。研究收集的蛋白质片段以鉴定候选蛋白质，并使用相对和绝对蛋白质定量（iTRAQ）的同量异位素标签进行相对定量。在这项研究中，共鉴定了378，078个光谱和3873个蛋白质。其中，2371个差异丰富蛋白（DAP）具有基因本体论术语，并且与来自京都基因和基因组百科全书的124个定义的途径相关。与花萼脱落有关的DAP主要参与光合作用、植物激素信号转导、细胞壁修饰和碳水化合物代谢。实时定量聚合酶链反应用于确认一些重要基因的表达模式。在分离的候选蛋白中，我们鉴定了表现出高度动态表达变化的蛋白，并且多聚半乳糖醛酸酶和几丁质酶似乎在花萼脱落过程中起关键作用。这些蛋白为进一步的功能鉴定和研究花萼脱落机制提供了重要的候选蛋白。本研究将加深对果实萼片脱落的生物学基础的认识，为提高梨的花萼脱落率和果实品质提供更多的参考。
'Kuerlexiangli' (Pyrus sinkiangensisYu) is an important market pear in China. The shape and the quality of fruits are negatively affected by the presence of a persistent calyx. To explore the molecular mechanism of calyx abscission, different protein expressions were identified at two critical processes of the calyx abscission under three treatments: an abscising treatment (6000 x Flusilazole + 300 x PBO), a persisting treatment (50 mg/L GA(3)) and a water control. The collected protein fragments were investigated to identify candidate proteins and perform relative quantification using isobaric tags for relative and absolute protein quantitation (iTRAQ). In this study, a total of 378,078 spectra and 3873 proteins were identified. Among them, 2371 differentially abundant proteins (DAPs) had Gene Ontology terms and were related to 124 defined pathways from the Kyoto Encyclopedia of Genes and Genomes. Some important DAPs relating to calyx abscission were mainly involved in photosynthesis, plant hormone signal transduction, cell-wall modification, and carbohydrate metabolism. Quantitative real-time polymerase chain reaction was used to confirm the expression patterns of some important genes. Among the isolated candidate proteins, we identified the proteins that exhibited highly dynamic expression changes and polygalacturonase and chitinase appeared to play key roles during the process of calyx abscission. These proteins are important candidate proteins for future functional identification and exploring the mechanism of calyx abscission. This study will enhance our knowledge of the biological basis of fruit sepal shedding, and provide more references for improving the calyx abscission rate and fruit quality of pear fruit.