Preparation of chitosan scaffolds with a hierarchical porous structure
Preparation of chitosan scaffolds with a hierarchical porous structure
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DOI：
10.1002/jbm.b.31586
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发表时间：
2010-05-01
影响因子：
3.4
通讯作者：
Chen, Guoping
中科院分区：
文献类型：
--
作者：
Ko, Young-Gwang;Kawazoe, Naoki;Chen, Guoping
组织工程需要开发具有开放表面孔结构的多孔支架，将细胞输送到支架的三维空间中，改善细胞分布。本研究以冰颗粒为模板制备了一种新型的漏斗状壳聚糖海绵，该漏斗状壳聚糖海绵具有层叠的双层多孔结构，表层和连通的大块多孔层，顶部表面多孔层主要由大孔层位于表面大孔下方，由与表面大孔相连的小孔组成，表面大孔取决于压纹冰颗粒的形状、尺寸和密度；壳聚糖漏斗状海绵表面开放的大孔和相互连接的大孔有利于细胞的播种和细胞从表面向内部大孔的分布，在漏斗状壳聚糖海绵中培养的细胞具有高活力、高增殖和均匀的组织形成，这种漏斗状壳聚糖海绵在组织工程中具有重要的应用价值(C) 2010 Wiley期刊。中国生物医学工程学报（英文版），2010
Development of porous scaffolds with open surface pore structures is required for tissue engineering to deliver cells into the three-dimensional spaces in the scaffolds and improve cell distribution This study demonstrated a new type of funnel-like chitosan sponge prepared using ice particulates as a template The funnel-like chitosan sponges had a hierarchical bilayer porous structure of a surface layer and an interconnected bulk porous layer The top surface porous layer consisted mainly of large open pores The bulk porous layer was beneath the large surface pores and consisted of small pores that were connected with the large surface pores The large surface pores were dependent on the shape, dimension, and den-say of the embossing ice particulates, while the bulk pores were dependent on the freezing temperature The large open surface pores and interconnected bulk pores in the funnel-like chitosan sponges facilitated cell seeding and cell distribution from the surface into the inner bulk pores Cells cultured in the funnel-like chitosan sponges showed high viability, high proliferation, and homogenous tissue formation Such funnel-like chitosan sponges will be useful for tissue engineering (C) 2010 Wiley Periodicals, Inc J Biomed Mater Res Part B Appl Biomater 93B 341-350, 2010