Response of soil bacterial communities to lead and zinc pollution revealed by Illumina MiSeq sequencing investigation
Response of soil bacterial communities to lead and zinc pollution revealed by Illumina MiSeq sequencing investigation
复制标题
Illumina MiSeq 测序研究揭示土壤细菌群落对铅和锌污染的响应
DOI：
10.1007/s11356-016-7826-3
复制
发表时间：
2017
影响因子：
5.8
通讯作者：
Shen Zhenguo
中科院分区：
文献类型：
--
作者：
Xu Xihui;Zhang Zhou;Hu Shunli;Ruan Zhepu;Jiang Ji;ong;Chen Chen;Shen Zhenguo
土壤为微生物群落的发育提供了重要的环境。然而，微生物可能对重金属（HMs）等物质敏感，这些物质是常见的土壤污染物。本研究采用16S核糖体RNA（rRNA）基因片段测序的地理区域和没有HM污染的细菌群落，以阐明土壤性质和HM对细菌群落的影响。在矿区和对照区的样品之间没有观察到细菌群落的丰富度或多样性的明显变化。不同地理区域的土壤细菌丰度和多样性存在显著差异，表明土壤基本特性是影响土壤细菌群落的最重要因素。然而，几个门和属的丰度显着不同的采矿和对照样品，表明锌和铅污染可能会影响土壤细菌群落组成。回归分析表明，各门属的相对丰度与土壤有效态Zn、Pb含量呈显著正相关。土壤钾、氨态氮、全铜、有效锌和有效铜含量是影响土壤肥力的主要因素。这些结果不仅表明土壤细菌对重金属胁迫敏感，而且还表明其他土壤性质可能在更大程度上影响土壤微生物。
Soil provides a critical environment for microbial community development. However, microorganisms may be sensitive to substances such as heavy metals (HMs), which are common soil contaminants. This study investigated bacterial communities using 16S ribosomal RNA (rRNA) gene fragment sequencing in geographic regions with and without HM pollution to elucidate the effects of soil properties and HMs on bacterial communities. No obvious changes in the richness or diversity of bacterial communities were observed between samples from mining and control areas. Significant differences in bacterial richness and diversity were detected between samples from different geographic regions, indicating that the basic soil characteristics were the most important factors affecting bacterial communities other than HMs. However, the abundances of several phyla and genera differed significantly between mining and control samples, suggesting that Zn and Pb pollution may impact the soil bacterial community composition. Moreover, regression analyses showed that the relative abundances of these phyla and genera were correlated significantly with the soil-available Zn and Pb contents. Redundancy analysis indicated that the soil K, ammoniacal nitrogen (NH4+–N), total Cu, and available Zn and Cu contents were the most important factors. Our results not only suggested that the soil bacteria were sensitive to HM stresses but also indicated that other soil properties may affect soil microorganisms to a greater extent.