Involvement of Tn4430 in transfer of Bacillus anthracis plasmids mediated by Bacillus thuringiensis plasmid pXO12
Involvement of Tn4430 in transfer of Bacillus anthracis plasmids mediated by Bacillus thuringiensis plasmid pXO12
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Tn4430参与苏云金芽孢杆菌质粒pXO12介导的炭疽芽孢杆菌质粒转移
DOI：
10.1128/jb.171.1.104-113.1989
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发表时间：
1989
影响因子：
3.2
通讯作者：
C. B. Thorne
中科院分区：
文献类型：
--
作者：
Brian D. Green;L. Battisti;C. B. Thorne
从苏云金芽孢杆菌亚种4042a菌株中分离得到的自传质粒pXO12，全长112.5 kb。苏云金杆菌，编码产生杀虫晶体蛋白(Cry+)。通过监测四环素抗性质粒pBC16(4.2kb)与炭疽芽孢杆菌毒素和囊质粒pXO1(168kb)和pXO2(85.6kb)的共转移，研究了pXO12介导的质粒转移的机制。炭疽杆菌供者与炭疽杆菌和蜡样芽孢杆菌受体配对后，TCR转染率为4.8×10(4)~3.9×10(6)/ml(频率为1.6×10(-4)~7.1×10(-2))，其中0.3%~0.4%同时遗传pXO1或pXO2。对转移载体的物理分析表明，pBC16是通过捐献过程转移的，而大的炭疽杆菌质粒是通过传导过程转移的。PXO1和pXO2的转移涉及Tn4430从pXO12转座到这些质粒上。DNA-DNA杂交实验证明，Tn4430位于pXO12的16.0kb的AvAI片段上。对pXO12的Tra-和Cry-衍生物的检测表明，该片段还含有与晶体形成有关的信息，并与含有携带配偶转移所需信息的DNA序列的限制性片段相邻。
The self-transmissible plasmid pXO12 (112.5 kilobases [kb]), originally isolated from strain 4042A of Bacillus thuringiensis subsp. thuringiensis, codes for production of the insecticidal crystal protein (Cry+). The mechanism of pXO12-mediated plasmid transfer was investigated by monitoring the cotransfer of the tetracycline resistance plasmid pBC16 (4.2 kb) and the Bacillus anthracis toxin and capsule plasmids, pXO1 (168 kb) and pXO2 (85.6 kb), respectively. In matings of B. anthracis donors with B. anthracis and Bacillus cereus recipients, the number of Tcr transcipients ranged from 4.8 x 10(4) to 3.9 x 10(6)/ml (frequencies ranged from 1.6 x 10(-4) to 7.1 x 10(-2), and 0.3 to 0.4% of them simultaneously inherited pXO1 or pXO2. Physical analysis of the transferred plasmids suggested that pBC16 was transferred by the process of donation and that the large B. anthracis plasmids were transferred by the process of conduction. The transfer of pXO1 and pXO2 involved the transposition of Tn4430 from pXO12 onto these plasmids. DNA-DNA hybridization experiments demonstrated that Tn4430 was located on a 16.0-kb AvaI fragment of pXO12. Examination of Tra- and Cry- derivatives of pXO12 showed that this fragment also harbored information involved in crystal formation and was adjacent to a restriction fragment containing DNA sequences carrying information required for conjugal transfer.
枯草芽孢杆菌与粪链球菌转座子 Tn917 的遗传转座和插入诱变。
DOI：
10.1073/pnas.80.8.2305
发表时间：
1983
影响因子：
11.1
作者：
Youngman,PJ;Perkins,JB;Losick,R
通讯作者：
Losick,R