IMMUNOCYTOCHEMICAL EVIDENCE OF LISTERIA, ESCHERICHIA-COLI, AND STREPTOCOCCUS ANTIGENS IN CROHNS-DISEASE
IMMUNOCYTOCHEMICAL EVIDENCE OF LISTERIA, ESCHERICHIA-COLI, AND STREPTOCOCCUS ANTIGENS IN CROHNS-DISEASE
复制标题
DOI：
10.1016/0016-5085(95)90687-8
复制
发表时间：
1995-05-01
期刊：
影响因子：
29.4
通讯作者：
COLOMBEL, JF
中科院分区：
文献类型：
--
作者：
LIU, Y;VANKRUININGEN, HJ;COLOMBEL, JF
背景/目的：长期以来，人们一直怀疑传染源在克罗恩病的引发中发挥了作用。本研究的目的是利用免疫细胞化学技术在患病组织中寻找可能的微生物制剂。方法：对来自康涅狄格州两个克罗恩病高发法国家庭的 21 名患者的肠道和肠系膜淋巴结标本进行了研究。搜索的微生物包括普通拟杆菌、伯氏疏螺旋体、大肠杆菌、单核细胞增生李斯特菌、链球菌、牛病毒性腹泻病毒、甲型流感病毒、麻疹病毒、副流感病毒和呼吸道合胞病毒。结果：75% 的克罗恩病患者（16 名中的 12 名）的李斯特菌抗体呈阳性标记。对该抗原进行免疫标记的巨噬细胞和巨细胞分布在溃疡下方、裂隙沿线、脓肿周围、固有层内、肉芽肿中以及肠系膜淋巴结的生发中心。此外，57% 的病例（21 例中的 12 例）含有大肠杆菌抗原，44% 的病例（16 例中的 7 例）含有链球菌抗原。后两种试剂的免疫标记也发生在巨噬细胞和巨细胞内，其分布模式与李斯特菌抗原相似。结论：结果表明，李斯特菌属、大肠杆菌和链球菌，而非麻疹病毒，在克罗恩病的发病机制中发挥作用。
Background/Aims: Infectious agents have long been suspected of playing a role in the initiation of Crohn's disease. The objective of this study was to search for likely microbial agents in diseased tissues using immunocytochemical techniques. Methods: Intestines and mesenteric lymph node specimens of 21 patients from two French families with a high frequency of Crohn's disease and from Connecticut were studied. The microbial agents searched for included Bacteroides vulgatus, Borrelia burgdorferi, Escherichia coli, Listeria monocytogenes, Streptococcus spp., bovine viral diarrhea virus, influenza A virus, measles virus, parainfluenza virus, and respiratory syncytial virus. Results: Seventy-five percent of the patients with Crohn's disease (12 of 16) were positively labeled with the antibody to Listeria. Macrophages and giant cells immunolabeled for this antigen were distributed underneath ulcers, along fissures, around abscesses, within the lamina propria, in granulomas, and in the germinal centers of mesenteric lymph nodes. In addition, 57% (12 of 21) of the cases contained the E. coli antigen, and 44% (7 of 16) contained the streptococcal antigen. The immunolabeling for the latter two agents also occurred within macrophages and giant cells, distributed in a pattern similar to that of Listeria antigen. Conclusions: The results suggest that Listeria spp., E. coli, and streptococci, but not measles virus, play a role in the pathogenesis of Crohn's disease.