Activation of apoptotic protein in U937 cells by a component of turmeric oil
Activation of apoptotic protein in U937 cells by a component of turmeric oil
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DOI：
10.5483/bmbrep.2009.42.2.096
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发表时间：
2009-02-28
期刊：
影响因子：
3.8
通讯作者：
Lee, Yongkyu
中科院分区：
文献类型：
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作者：
Lee, Yongkyu
来自姜黄油的芳香族（ar）-姜黄酮显示包括抑制细胞增殖的抗肿瘤发生活性。本研究探讨了在人淋巴瘤U937细胞中，姜姜酮介导的凋亡蛋白激活。姜黄酮处理以浓度依赖性方式抑制U937细胞活力，抑制率超过84%。此外，处理产生核小体DNA片段，亚二倍体细胞的百分比以浓度依赖性方式增加;两者都是细胞凋亡的标志。ar-tunnerone的凋亡作用与Bax和p53蛋白的诱导相关，而不是Bcl-2和p21。线粒体细胞色素c和caspase-3的激活表明caspase-3的激活伴随着ar-turmerone的凋亡作用，其介导细胞死亡。这些结果表明，ar-tunnerone对U937细胞的凋亡作用可能涉及通过诱导Bax和p53激活caspase-3，而不是Bcl-2和p21。[BMB报告2009; 42（2）：96-100]
Aromatic (ar)-turmerone from turmeric oil displays anti-tumorigenesis activity that includes inhibited cell proliferation. This study investigated ar-turmerone-mediated apoptotic protein activation in human lymphoma U937 cells. Ar-turmerone treatment inhibited U937 cell viability in a concentration-dependent fashion, with inhibition exceeding 84%. Moreover, the treatment produced nucleosomal DNA fragmentation and the percentage of sub-diploid cells increased in a concentration-dependent manner; both are hallmarks of apoptosis. The apoptotic effect of ar-tunnerone was associated with the induction of Bax and p53 proteins, rather than Bcl-2 and p21. Activation of mitochondrial cytochrome c and caspase-3 demonstrated that the activation of caspases accompanied the apoptotic effect of ar-turmerone, which mediated cell death. These results suggest that the apoptotic effect of ar-tunnerone on U937 cells may involve caspase-3 activation through the induction of Bax and p53, rather than Bcl-2 and p21. [BMB reports 2009; 42(2): 96-100]