Selective Gene Delivery for Integrating Exogenous DNA into Plastid and Mitochondrial Genomes Using Peptide-DNA Complexes.
Selective Gene Delivery for Integrating Exogenous DNA into Plastid and Mitochondrial Genomes Using Peptide-DNA Complexes.
复制标题
使用肽-DNA 复合物选择性基因递送将外源 DNA 整合到质体和线粒体基因组中。
作者：
T. Yoshizumi;Kazusato Oikawa;Jo;Y. Kodama;K. Numata
由于缺乏适当的平台技术，选择性地将基因输送到细胞器基因组(线粒体和叶绿体基因组)一直是有限的，尽管这些细胞器对于代谢产物和能量的产生是必不可少的。细胞器的选择性修饰技术是提高细胞器功能和产生转体/传递线粒体植物所必需的。然而，目前还没有针对包括植物在内的多细胞生物建立线粒体基因组修饰的方法。同样，对叶绿体基因组的修改也仅限于少数几种植物和藻类。在本研究中，我们开发了含有细胞器靶向信号的融合肽与质粒DNA的离子络合物，用于选择性地将外源DNA转移到完整植物的叶绿体和线粒体基因组中。这是首次报道外源DNA不仅整合到植物的线粒体基因组中，而且整合到一般的多细胞生物的线粒体基因组中。这种融合多肽介导的基因递送系统是植物细胞器生物技术和动物线粒体疾病基因治疗的一个突破性平台。
Selective gene delivery into organellar genomes (mitochondrial and plastid genomes) has been limited because of a lack of appropriate platform technology, even though these organelles are essential for metabolite and energy production. Techniques for selective organellar modification are needed to functionally improve organelles and produce transplastomic/transmitochondrial plants. However, no method for mitochondrial genome modification has yet been established for multicellular organisms including plants. Likewise, modification of plastid genomes has been limited to a few plant species and algae. In the present study, we developed ionic complexes of fusion peptides containing organellar targeting signal and plasmid DNA for selective delivery of exogenous DNA into the plastid and mitochondrial genomes of intact plants. This is the first report of exogenous DNA being integrated into the mitochondrial genomes of not only plants, but also multicellular organisms in general. This fusion peptide-mediated gene delivery system is a breakthrough platform for both plant organellar biotechnology and gene therapy for mitochondrial diseases in animals.