Quercetin regulates Th1/Th2 balance in a murine model of asthma
Quercetin regulates Th1/Th2 balance in a murine model of asthma
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DOI：
10.1016/j.intimp.2008.10.021
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发表时间：
2009-03-01
影响因子：
5.6
通讯作者：
Park, Yeong-Min
中科院分区：
文献类型：
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作者：
Park, Hee-ju;Lee, Chang-Min;Park, Yeong-Min
研究发现槲皮素是黄酮类化合物中最具活性的，许多药用植物的活性很大程度上归功于其高槲皮素含量。槲皮素已经证明了显着的抗炎活性，因为直接抑制炎症的几个初始过程。然而，其在Th 1/Th 2免疫应答中的抗过敏作用知之甚少。最近研究表明，T-bet和加塔-3是Th 1和Th 2的主要调节转录因子。本研究旨在探讨槲皮素对哮喘小鼠Th 1/Th 2细胞因子产生、T-bet和加塔-3基因表达的调节作用，以及槲皮素对哮喘小鼠IL-4、Th 2细胞因子产生的抑制作用。槲皮素还能增加IFN-γ、Th 1细胞因子的产生。我们还研究了槲皮素是否可以影响嗜酸性粒细胞过氧化物酶（EPO）的活性。在最后一次气道OVA激发前给予槲皮素导致对所有哮喘反应的显著抑制。因此，本研究为槲皮素在改善小鼠哮喘发病过程中发挥重要作用提供了证据。这些研究结果提供了新的见解槲皮素的免疫药理学作用方面的影响，在小鼠哮喘模型，也拓宽了目前的观点，在我们的理解槲皮素的免疫药理学功能。(C)2009 Elsevier B. V.保留所有权利。
Quercetin is found to be the most active of the flavonoids in studies and many medicinal plants owe much of their activity to their high Quercetin content. Quercetin has demonstrated significant anti-inflammatory activity because of direct inhibition of several initial processes of inflammation. However, its anti-allergic effect in the Th1/Th2 immune response was poorly understood. Recently, it was shown that T-bet and GATA-3 were master Th1 and Th2 regulatory transcription factors. In this study, we have attempted to determine whether Quercetin regulates Th1/Th2 cytokine production, T-bet and GATA-3 gene expression in OVA-induced asthma model mice.Quercetin reduced the increased levels of IL-4, Th2 cytokine production in OVA-sensitized and -challenged mice. The other side, it increased IFN-gamma, Th1 cytokine production in Quercetin administrated mice. We also examined to ascertain whether Quercetin could influence Eosinophil peroxidase (EPO) activity. The administration of Quercetin before the last airway OVA challenge resulted in a significant inhibition of all asthmatic reactions. Accordingly, this study may provide evidence that Quercetin plays a critical role in the amelioration of the pathogenetic process of asthma in mice. These findings provide new insight into the immunopharmacological role of Quercetin in terms of its effects in a murine model of asthma, and also broaden current perspectives in our understanding of the immunopharmacological functions of Quercetin. (C) 2009 Elsevier B.V. All rights reserved.