Both gravistimulation onset and removal trigger an increase of cytoplasmic free calcium in statocytes of roots grown in microgravity.
Both gravistimulation onset and removal trigger an increase of cytoplasmic free calcium in statocytes of roots grown in microgravity.
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DOI：
10.1038/s41598-018-29788-7
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发表时间：
2018-07-30
影响因子：
4.6
通讯作者：
Legué V
中科院分区：
文献类型：
--
作者：
Bizet F;Pereda-Loth V;Chauvet H;Gérard J;Eche B;Girousse C;Courtade M;Perbal G;Legué V
重力是引导植物生长发育的永久性环境信号。植物的重力感应始于被称为平衡石的充满淀粉的质体的位移，最终导致生长素的重新分布和器官的弯曲。虽然参与重力感应的几个演员，如钙是已知的，平衡石位移对钙变化的影响仍然是谜。微重力是一种独特的环境条件，为破译这种联系提供了机会。在这项研究中，甘蓝型油菜的根在国际空间站（ISS）上在微重力或模拟地球重力的离心机中生长。短模拟重力的开始和去除的影响进行了测量平衡石定位和细胞内游离钙进行了评估，使用焦锑酸盐沉淀作为细胞溶质钙标记。我们的研究结果表明，10分钟的发病或重力的去除引起非常低的耳石位移，但这是，尽管如此，与焦锑酸盐沉淀物的数量增加。这些结果突出表明，细胞溶质钙分布的变化是在没有一个显着的平衡石位移触发。
Gravity is a permanent environmental signal guiding plant growth and development. Gravity sensing in plants starts with the displacement of starch-filled plastids called statoliths, ultimately leading to auxin redistribution and organ curvature. While the involvement in gravity sensing of several actors such as calcium is known, the effect of statolith displacement on calcium changes remains enigmatic. Microgravity is a unique environmental condition offering the opportunity to decipher this link. In this study, roots of Brassica napus were grown aboard the International Space Station (ISS) either in microgravity or in a centrifuge simulating Earth gravity. The impact of short simulated gravity onset and removal was measured on statolith positioning and intracellular free calcium was assessed using pyroantimonate precipitates as cytosolic calcium markers. Our findings show that a ten-minute onset or removal of gravity induces very low statolith displacement, but which is, nevertheless, associated with an increase of the number of pyroantimonate precipitates. These results highlight that a change in the cytosolic calcium distribution is triggered in absence of a significant statolith displacement.
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影响因子：
4.6
作者：
Chauvet H;Pouliquen O;Forterre Y;Legué V;Moulia B
通讯作者：
Moulia B
影响因子：
6.4
作者：
IVERSEN, TH;PEDERSEN, K;LARSEN, P
通讯作者：
LARSEN, P
影响因子：
4.3
作者：
SACK, FD;SUYEMOTO, MM;LEOPOLD, AC
通讯作者：
LEOPOLD, AC
影响因子：
6.4
作者：
Driss-Ecole, Dominique;Legue, Valerie;Perbal, Gerald
通讯作者：
Perbal, Gerald
影响因子：
6.9
作者：
IVERSEN, TH;ROMMELHOFF, A
通讯作者：
ROMMELHOFF, A