Simultaneous determination of Zilpaterol and other beta agonists in calf eye by gas chromatography/tandem mass spectrometry.
Simultaneous determination of Zilpaterol and other beta agonists in calf eye by gas chromatography/tandem mass spectrometry.
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DOI：
10.1093/jaoac/86.1.8
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发表时间：
2003
影响因子：
1.6
通讯作者：
B. Bocca;M. Fiori;C. Cartoni;G. Brambilla
中科院分区：
文献类型：
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作者：
B. Bocca;M. Fiori;C. Cartoni;G. Brambilla
在欧洲肉类生产中，用于促进生长的肾上腺素能药物已被禁止;然而，在美国，墨西哥和南非，莱克多巴胺和齐帕特罗等分子被许可用于饲养猪和牛。最近对牛视网膜提取物的分析显示，停药后的检测期显著延长。先前的研究表明，在停药第50天，视网膜组织中克伦特罗和相关物质的残留浓度> 100 ng/g。开发了一种方法，以确定和同时定量克伦特罗样物质与苯胺部分和药物与酚和儿茶酚部分，如莱克多巴胺和齐帕特罗，在视网膜组织。根据SANCO/1805/2000对该方法进行了验证。在0.1 N HCl中萃取后，在C18非封端固相萃取柱上对样品进行净化，并通过气相色谱/串联质谱法（电子轰击模式）分析三甲基氯硅烷衍生物。在牛视网膜中，激动剂浓度在62.5 - 250.0 ng/g之间时，平均回收率范围为85.3 - 94.8%，重复性<9.6%，实验室内重现性<10.5%。决定限（CCalpha）范围为66.3-70.4 ng/g，检测能力（CCbeta）范围为73.9 - 79.8 ng/g。结果进行了讨论方面的多残留的方法，以提高可靠性的监测策略。
Adrenergic drugs for growth promotion have been outlawed in European meat production; however, molecules such as Ractopamine and Zilpaterol are licensed for feeding swine and cattle in the United States, Mexico, and South Africa. Analysis of bovine retinal extracts has recently shown considerable extension in the detection period following withdrawal. Previous studies demonstrated that residual concentrations of Clenbuterol and related substances in retinal tissue were > 100 ng/g at day 50 of withdrawal. A method was developed to identify and simultaneously quantify Clenbuterol-like substances with anilinic moieties and drugs with phenolic and catecholic moieties, such as Ractopamine and Zilpaterol, in retinal tissue. The method was validated according to SANCO/1805/2000. After extraction in 0.1 N HCl, samples were cleaned up on C18 non-endcapped solid-phase extraction columns and analyzed as trimethylchlorosilane derivatives by gas chromatography/tandem mass spectrometry, electron impact mode. At concentrations of agonists between 62.5 and 250.0 ng/g in bovine retina, mean recoveries ranged from 85.3 to 94.8%, repeatability was < 9.6%, and within-laboratory reproducibility was < 10.5%. The decision limits (CCalpha) were within the range of 66.3-70.4 ng/g, and the detection capability (CCbeta) varied from 73.9 to 79.8 ng/g. Results are discussed in terms of a multiresidue approach to improve reliability of the monitoring strategy.