A novel human prostate-specific, androgen-regulated homeobox gene (NKX3.1) that maps to 8p21, a region frequently deleted in prostate cancer
A novel human prostate-specific, androgen-regulated homeobox gene (NKX3.1) that maps to 8p21, a region frequently deleted in prostate cancer
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DOI：
10.1006/geno.1997.4715
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发表时间：
1997-07-01
期刊：
影响因子：
4.4
通讯作者：
Carter, KC
中科院分区：
文献类型：
--
作者：
He, WW;Sciavolino, PJ;Carter, KC
我们已经在人类中分离了前列腺特异性基因（NKX3.1），该基因与果蝇NK同源基因家族同源，Northern印迹分析表明，该基因在成人前列腺中以高水平表达，并且在睾丸水平较低，睾丸，睾丸水平较低。但在其他几个组织中几乎没有表达。在雄激素依赖性的前列腺癌系中，LNCAP，nkx3.1 mRNA以基础水平表达，在雄激素刺激后显着增加。 NKX3.1 mRNA在其他几种人类肿瘤细胞系中是无法检测的，包括两个独立于雄激素的前列腺癌。 NKX3.1基因映射到染色体带8P21，该区域经常据报道会在前列腺癌进展过程中与组织去分化和雄激素反应性丧失相关的杂合性丧失。基于这些数据，我们建议NKX3.1是在前列腺癌进展过程中以雄激素驱动的前列腺组织分化和分化的损失的相反过程中发挥作用的候选基因。 （c）1997学术出版社。
We have isolated a prostate-specific gene (NKX3.1) in humans that is homologous to the Drosophila NK homeobox gene family, Northern blot analyses indicate that this gene is expressed at high levels in adult prostate and at a much lower level in testis, but is expressed little or not at all in several other tissues. In an androgen-dependent prostate carcinoma line, LNCaP, NKX3.1 mRNA is expressed at a basal level that was increased markedly upon androgen stimulation; the NKX3.1 mRNA was undetectable in several other human tumor cell lines including two androgen-independent prostate carcinoma lints. The NKX3.1 gene maps to chromosome band 8p21, a region frequently reported to undergo a loss of heterozygosity associated with tissue dedifferentiation and loss of androgen responsiveness during the progression of prostate cancer. Based on these data we propose that NKX3.1 is a candidate gene for playing a role in the opposing processes of androgen-driven differentiation of prostatic tissue and loss of that differentiation during the progression of prostate cancer. (C) 1997 Academic Press.