Muscarinic cholinergic inhibition of adenylate cyclase in airway smooth muscle.
Muscarinic cholinergic inhibition of adenylate cyclase in airway smooth muscle.
复制标题
气道平滑肌中腺苷酸环化酶的毒蕈碱胆碱能抑制。
DOI：
10.1152/ajpcell.1987.253.1.c97
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Brown,JK
中科院分区：
文献类型：
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作者：
Jones,CA;Madison,JM;Tom-Moy,M;Brown,JK
本研究的目的是测试乙酰胆碱对犬气管肌腺苷酸环化酶活性的抑制作用。因此，细胞从肌肉中以酶法分散和裂解，并在从裂解物中分离的膜悬浮液中检测腺苷环化酶的活性。在GTP(10(-4)M)存在的情况下，最大的β-肾上腺素能刺激使腺苷环化酶的活性比单独使用GTP时增加一倍。在GTP存在下，乙酰胆碱(10(-4)M)使活性从97+/-21降至55+/-13pmolcAMP X min-1X mg蛋白-1(Means+/-SE；n=5；P<0.05)，在GTP+异丙肾上腺素(10(-4)M)存在下，乙酰胆碱引起的活性下降从163+/-29降至101+/-15pmolcAMP X min-1X mg蛋白-1(P<0.05)。这些减少是剂量依赖的，并被一系列胆碱能药物以与毒鼠碱效应一致的模式改变。我们的结果提示，迷走神经刺激犬中央呼吸道的一种生化效应可能是降低了平滑肌中腺苷环化酶的活性。
The goal of our study was to test for an inhibitory effect of acetylcholine on adenylate cyclase activity in canine trachealis muscle. Therefore, cells were dispersed from the muscle enzymatically and lysed, and adenylate cyclase activity was assayed in a membrane suspension isolated from the lysates. Maximal beta-adrenergic stimulation, in the presence of GTP (10(-4) M), increased the activity of adenylate cyclase twofold above the activity induced by GTP alone. In the presence of GTP, acetylcholine (10(-4) M) decreased activity from 97 +/- 21 to 55 +/- 13 pmol cyclic AMP X min-1 X mg protein-1 (means +/- SE; n = 5; P less than 0.05); in the presence of GTP plus isoproterenol (10(-4) M), the acetylcholine-induced decreases were from 163 +/- 29 to 101 +/- 15 pmol cyclic AMP X min-1 X mg protein-1 (P less than 0.05). These decreases were dose dependent and they were altered by a series of cholinergic agents in a pattern consistent with a muscarinic effect. Our results suggest that one biochemical effect of vagal stimulation in the central airways of dogs may be attenuated adenylate cyclase activity in the smooth muscle.