Pulsed flow modulation comprehensive two-dimensional gas chromatography
Pulsed flow modulation comprehensive two-dimensional gas chromatography
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DOI：
10.1016/j.chroma.2007.09.030
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发表时间：
2008-04-04
影响因子：
4.1
通讯作者：
Amirav, Aviv
中科院分区：
文献类型：
--
作者：
Poliak, Marina;Kochman, Maya;Amirav, Aviv
脉冲流量调制（PFM）基于第一个GC柱流出物的较高流速时间压缩，其在注入第二个柱之前在标准熔融石英宽孔传输线中存储几秒钟。我们用两个标准的1/16 in.黄铜压缩配件，将两个柱插入0.53 mm W的宽孔内。熔融石英存储传输线，用于消除死体积。这种简单的布置提供了样品存储传输线长度的灵活性，从而提供了全面的二维气相色谱（GC x GC）循环时间、惰性样品路径和完全消除冷却气体消耗的组合。证明了创纪录的20 ms短第二柱进样时间。实际的进样时间是样品收集时间（例如4秒）除以第二柱与第一柱的流速比（例如20/0.7），其通常为约150 ms。由于装置的低成本，也可以考虑与非全面的时间分段GC x GC一起使用，以去除一些意外的共洗脱。PFM-GC x GC由于使用0.32 mm i.d.具有高流速的柱作为第二维GC x GC柱。因此，PFM-GC x GC的第二柱进样容量和线性动态范围可高出两个数量级，从而更好地减少由于柱过载而产生的不利基质干扰效应。(C)2007 Elsevier B. V.保留所有权利。
Pulsed flow modulation (PFM) is based on higher flow rate time compression of the first GC column effluent, which prior to the injection into the second column is stored for a few seconds in a standard fused silica wide bore transfer line. We constructed the PFM device with two standard 1/16 in. brass compression fittings with the insertion of the two columns inside the wide bore 0.53 mm W. fused silica storage transfer line for the elimination of dead volumes. This simple arrangement provides a combination of flexibility in the length of the sample storage transfer line hence comprehensive two-dimensional gas chromatography (GC x GC) cycle time, inert sample path and full elimination of cooling gas consumption. A record short second column injection time of 20 ms is demonstrated. Practical injection times are the sample collection time (such as 4 s) divided by the second to first column flow rate ratio (such as 20/0.7), which is typically around 150 ms. Due to the low cost of the device it can also be considered for use with non comprehensive time segmented GC x GC to remove a few accidental coelutions. PFM-GC x GC excels with high second column capacity due to the use of 0.32 mm i.d. columns with high flow rates as the second dimension GC x GC column. As a result, PFM-GC x GC can have up to two orders of magnitude higher second column sample capacity and linear dynamic range for improved reduction of adverse matrix interference effects due to column overloading. (C) 2007 Elsevier B.V. All rights reserved.