Stoichiometry in zinc ion transfer from metallothionein to zinc finger peptides
Stoichiometry in zinc ion transfer from metallothionein to zinc finger peptides
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DOI：
10.1016/s1387-3806(00)00343-2
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发表时间：
2001-02-06
影响因子：
1.8
通讯作者：
Fenselau, C
中科院分区：
文献类型：
--
作者：
Hathout, Y;Fabris, D;Fenselau, C
采用电喷雾和纳米喷雾电离技术研究了锌离子在锌-7-金属硫蛋白和载脂蛋白肽之间的转移，载脂蛋白肽是几种锌指蛋白的模型。这里报道的膜实验表明，这些转移所需的蛋白质间接触。在四极离子阱上的分析允许同时监测所有反应物和所有产物。证据提供了一个单一的锌离子从每个Zn-7-金属硫蛋白复合物的优先转移。(C)2001 Elsevier Science B.V.
Electrospray and nanospray ionizations are used to study the transfer of zinc ions between Zn-7-metallothionein and apo peptides that are models for several kinds of zinc finger proteins. A membrane experiment is reported here which demonstrates that interprotein contact is required for these transfers. Analysis on a quadrupole ion trap has allowed all reactants and all products to be monitored simultaneously. Evidence is provided for the preferential transfer of a single zinc ion from each Zn-7-metallothionein complex. (C) 2001 Elsevier Science B.V.