Effect of temperature in long term preservation of vascular endothelial and smooth muscle function
Effect of temperature in long term preservation of vascular endothelial and smooth muscle function
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DOI：
10.1016/0003-4975(96)00109-9
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发表时间：
1996-05-01
影响因子：
4.6
通讯作者：
Steen, S
中科院分区：
文献类型：
--
作者：
Ingemansson, R;Budrikis, A;Steen, S
背景在临床移植中，供体器官用冷保存溶液灌注，以获得快速的核心冷却和适合组织细胞的环境。如果没有良好的脉管系统保护，再灌注期间血流的进行性恶化可能最终导致无复流现象，即使器官中其他细胞的功能可以得到充分保护。本研究的目的是找到最佳的存储温度保存的血管。在器官浴中研究了126只Sprague-Dawley大鼠的肾下主动脉：作为新鲜对照，在威斯康星州大学溶液中于0.5 ℃、4 ℃、8.5 ℃和22 ℃储存36小时后，以及储存36小时后移植和2小时、24小时和7天后。血栓素类似物U-46619用于测试收缩性。用乙酰胆碱诱发内皮依赖性舒张（EDR），罂粟碱诱发内皮非依赖性舒张。证明在0.5 ℃下储存血管在保持收缩性方面最好，没有显著降低，而在4 ℃和8.5 ℃下储存导致36小时后显著降低。在体内再灌注24小时内收缩性没有恢复，但7天后完全恢复。无论使用的保存温度如何，在储存36小时后观察到EDR的显著损害。移植后两小时，在4 ℃和8.5 ℃下储存的血管EDR没有明显受损，而在0.5 ℃下储存的血管EDR明显丧失。24 h后和7 d后，各组EDR均正常。内皮依赖性舒张因子功能在4 ℃和8.5 ℃下保存得最好，而血管平滑肌功能的保存在0.5 ℃下最好。
Background. In clinical transplantation the donor organ is perfused with a cold preservation solution to obtain quick core cooling and a suitable environment for the tissue cells. Without good preservation of the vasculature, progressive deterioration of the blood flow during reperfusion may ultimately lead to the no-reflow phenomenon, even though the function of the other cells in the organ may be adequately preserved. The aim of this study was to find the optimal storage temperature for preservation of the vasculature.Methods. The infrarenal aorta of 126 Sprague-Dawley rats were studied in organ baths: as fresh controls, after 36 hours of storage at 0.5 degrees C, 4 degrees C, 8.5 degrees C, and 22 degrees C in University of Wisconsin solution, and after 36-hour storage followed by transplantation and a lapse of 2 hours, 24 hours, and 7 days. The thromboxane analogue U-46619 was used to test contractility. Acetylcholine was used to elicit endothelium-dependent relaxation (EDR), and papaverine to elicit endothelium-independent relaxation.Results. Storing the vessels at 0.5 degrees C proved best regarding preservation of contractility, with a nonsignificant decrease, whereas storage at 4 degrees C and 8.5 degrees C resulted in a significant decrease after 36 hours. The contractility did not recover within 24 hours of in vivo reperfusion, but full recovery was seen after 7 days. Regardless of the preservation temperature used, a significant impairment in EDR was seen after 36 hours of storage. Two hours after transplantation, vessels stored at 4 degrees C and 8.5 degrees C showed no significant impairment in EDR, whereas those stored at 0.5 degrees C demonstrated a significant loss of EDR. After 24 hours and after 7 days, EDR was normal in all groups.Conclusions. Endothelium-dependent relaxing factor function is best preserved at 4 degrees C and 8.5 degrees C, whereas preservation of vascular smooth muscle function is best preserved at 0.5 degrees C.