Regulation of chondrogenesis by heparan sulfate and structurally related glycosaminoglycans.
Regulation of chondrogenesis by heparan sulfate and structurally related glycosaminoglycans.
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DOI：
10.1016/0012-1606(87)90422-2
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发表时间：
1987-09
影响因子：
2.7
通讯作者：
J. S. Antonio;B. M. Winston;R. Tuan
中科院分区：
文献类型：
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作者：
J. S. Antonio;B. M. Winston;R. Tuan
为了测试可能参与的硫酸乙酰肝素蛋白多糖在软骨形成，我们已经研究了其主要的糖胺聚糖（GAG）的组成部分，硫酸乙酰肝素，和其他结构相关的糖胺聚糖对软骨形成鸡肢芽间充质（Hamburger-Hamilton阶段23 - 24）微团培养的效果。发现硫酸乙酰肝素及其几种类似物（肝素、硫酸皮肤素和硫酸葡聚糖）可显著刺激软骨结节形成;此外，硫酸乙酰肝素和肝素也可促进结节生长。硫酸软骨素不刺激软骨形成。GAG的活性取决于其剂量、分子大小、电荷和化学结构。在来自第25阶段胚胎翅尖亚嵴中胚层的微团培养物中也观察到类似的效果，所述中胚层是软骨祖细胞的富集群体。GAG在培养过程中的作用时间表明它们参与软骨形成的细胞聚集后事件，例如软骨细胞表型的初始表达或软骨结节的生长。
To test the possible involvement of heparan sulfate proteoglycans in chondrogenesis, we have studied the effect of their major glycosaminoglycan (GAG) component, heparan sulfate, and other structurally related GAGs on chondrogenesis in micromass cultures of chick limb bud mesenchyme (Hamburger-Hamilton stages 23 24). Heparan sulfate and several of its analogs (heparin, dermatan sulfate, and dextran sulfate) were found to significantly stimulate cartilage nodule formation; in addition, heparan sulfate and heparin also promoted nodule growth. Chondroitin sulfate did not stimulate chondrogenesis. The activity of the GAGs was dependent on their dose, molecular size, charge, and chemical structure. Similar effects were also observed in micromass cultures derived from stage 25 embryonic wing-tip subridge mesoderm, an enriched population of chondroprogenitor cells. The time of action of the GAGs during culture suggested their involvement in post-cell aggregation events of chondrogenesis, such as the initial expression of the chondrocyte phenotype or the growth of cartilage nodules.