Rapid quantitation of Trypanosoma cruzi in host tissue by real-time PCR
Rapid quantitation of Trypanosoma cruzi in host tissue by real-time PCR
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DOI：
10.1016/s0166-6851(03)00093-8
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发表时间：
2003-06-01
影响因子：
1.5
通讯作者：
Tarleton, RL
中科院分区：
文献类型：
--
作者：
Cummings, KL;Tarleton, RL
一种实时聚合酶链式反应技术，允许准确和灵敏地量化感染原虫寄生虫克鲁兹锥虫的动物的组织寄生虫负担。是发展起来的。通过确认急性感染小鼠的寄生虫负荷高于慢性感染小鼠，检测不同寄生虫：宿主菌株组合中组织限制性寄生虫的持久性，以及观察感染剂量越高，组织寄生虫负荷增加，证明了该方法的有效性。该方法可作为监测不同治疗方案下宿主体内寄生虫负荷的有用工具。(C)2003 Elsevier Science B.V.保留所有权利。
A real-time PCR technique that allows for accurate and sensitive quantitation of tissue parasite burden in animals infected with the protozoan parasite Trypanosoma cruzi. was developed. The utility of this method was demonstrated by confirmation of higher parasite load in mice with acute infections in comparision to chronically infected mice, detection of tissue-restricted parasite persistence in different parasite:host strain combinations, and the observation of increased tissue parasite burden with higher infective doses. This method should be a useful tool for monitoring parasite burden in hosts under various treatment regimens. (C) 2003 Elsevier Science B.V. All rights reserved.