Induction of cytochrome P4501A by binary mixtures of polychlorinated biphenyls (PCBs) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in liver cell lines from rat and trout
Induction of cytochrome P4501A by binary mixtures of polychlorinated biphenyls (PCBs) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in liver cell lines from rat and trout
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DOI：
10.1016/s0166-445x(98)00048-4
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发表时间：
1998-10-01
影响因子：
4.5
通讯作者：
Bols, NC
中科院分区：
文献类型：
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作者：
Clemons, JH;Myers, CR;Bols, NC
在大鼠 (H4IIE) 和虹鳟鱼 (RTL-W1) 的肝细胞系中检测了 2,3,7,X-四氯二苯并-对二恶英 (TCDD) 和七种多氯联苯 (PCB) 的二元组合对乙氧基试卤灵-o-脱乙基酶 (EROD) 活性的诱导作用。非邻位 PCB 为 77、81、126 和 169；单邻位 PCB 为 105、118 和 156。与 H4IIE 相比，二元组合与 RTL-W1 更常见地产生低于加性的相互作用。在RTL-W1中，在单邻位PCB/TCDD比率大于10000比I和非邻位PCB/TCDD比率大于80比1时观察到小于加和行为。在H4IIE中，在单邻位PCB/TCDD比率大于20000比I时观察到小于加和相互作用，但仅在TCDD和非邻位PCB之间观察到加和相互作用。对于这两种细胞系，没有发现大于相加的相互作用。这些结果意味着这些细胞系的生物测定可能导致比化学分析测定的 TCDD 当量浓度更低。 (C) 1998 Elsevier Science B.V. 保留所有权利。
The induction of ethoxyresorufin-o-deethylase (EROD) activity by binary combinations of 2,3,7,X-tetrachlorodibenzo-p-dioxin (TCDD) and seven polychlorinated biphenyls (PCBs) was examined in liver cell lines from rat (H4IIE) and rainbow trout (RTL-W1). The non-ortho PCBs were 77, 81, 126, and 169; the mono-ortho PCBs were 105, 118, and 156. Binary combinations yielded less-than-additive interactions more commonly with RTL-W1 than with H4IIE. In RTL-W1, less-than-additive behaviour was observed at mono-ortho PCB/TCDD ratios of greater than 10000 to I and at non-ortho PCB/TCDD ratios of greater than 80 to 1. In H4IIE, less-than-additive interactions were observed at mono-ortho PCB/TCDD ratios of greater than 20000 to I, but only additive interactions were seen between TCDD and non-ortho PCBs. With both cell lines, greater-than-additive interactions were not found. These results imply that bioassays with these cell lines could lead to lower TCDD-equivalent concentrations than would be determined by chemical analysis. (C) 1998 Elsevier Science B.V. All rights reserved.