Genetics and electrophoretic karyotyping of wild-type and astaxanthin mutant strains of Phaffia rhodozyma
Genetics and electrophoretic karyotyping of wild-type and astaxanthin mutant strains of Phaffia rhodozyma
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DOI：
10.1023/a:1000200119447
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发表时间：
1997-08-01
影响因子：
2.6
通讯作者：
Jimenez, A
中科院分区：
文献类型：
--
作者：
Cifuentes, V;Hermosilla, G;Jimenez, A
在这项工作中，我们建立了一个野生型，一个虾青素和两个β -胡萝卜素过量生产的红酵母法菲红酵母菌的染色体组成。所使用的方法是脉冲场凝胶电泳，确定了酵母基因组中的9条DNA染色体带。两个最大的带是三重带，另外两个带，VI和VIII，似乎是双重带。染色体带的大小在0.35和2.5 Mb之间变化，表明基因组大小为25 Mb。所使用的技术，与使用特定DNA探针的杂交分析相补充，提供了关于P. rhodozyma基因组组织的直接信息。我们还克隆并定位了不同的DNA序列，这些DNA序列编码翻译延伸因子1 α (ef-1 α)， 7.6 kb的重复DNA BamHI片段（可能是rDNA）和随机选择的片段（命名为位点R2）。此外，我们已经检测到在我们实验室获得的影响胡萝卜素生成的野生型菌株和突变株之间的染色体长度多态性。
In this work we establish the chromosomal composition of a wild-type, one astaxanthin and two beta-carotene overproducer strains of the red yeast Phaffia rhodozyma. The method used has been pulsed field gel electrophoresis, which has determined 9 DNA chromosomal bands in the yeast genome. The two largest bands are triplets and two other bands, VI and VIII, seem to be doublets. The size of the chromosomal bands varies between 0.35 and 2.5 Mb, suggesting a genome size of 25 Mb. The technique used, complemented with hybridization assays using specific DNA probes, provides direct information about the genomic organization of P. rhodozyma. We have also cloned and located in chromosomal bands different DNA sequences that code for the translation elongation factor 1 alpha (ef-1 alpha), a 7.6 kb BamHI fragment of repetitive DNA (possibly rDNA) and a randomly chosen fragment (named locus R2). Additionally, we have detected a chromosomal length polymorphism between wild-type strains and mutant strains affecting carotenogenesis obtained in our laboratory.