Theoretical investigations of a novel microfluidic cooling/warming system for cell vitrification cryopreservation
Theoretical investigations of a novel microfluidic cooling/warming system for cell vitrification cryopreservation
复制标题
用于细胞玻璃化冷冻保存的新型微流体冷却/加温系统的理论研究
DOI：
10.1016/j.ijheatmasstransfer.2013.06.022
复制
发表时间：
2013-10-01
影响因子：
5.2
通讯作者：
Gao, Dayong
中科院分区：
文献类型：
--
作者：
Zhou, Xiaoming;Liu, Zhong;Gao, Dayong
超快速冷却和复温是玻璃化冷冻保存的必要条件。在这项工作中，现有的冷却/升温方法进行了审查，然后提出了一种新的微流体系统。设计的主要考虑包括：（a）利用芯片表面的微通道阵列来提高外部传热系数;（B）通过将样品作为超薄膜保持在系统内部来增加样品表面与其体积的关系。通过交替使用液氮或温水作为其工作流体，该系统可以用于冷却和加热。为了评估其在实现超快速冷却/升温速率方面的应用，从理论上研究了冷却和升温过程中外部传热系数h的大小，然后对样品溶液的瞬态温度分布、冷却/升温速率和玻璃化/失透趋势进行了数值研究。结果表明，微流控系统在制冷和升温过程中都能获得超高h（>10（4）W/m（2）K），有效地提高了制冷/升温速率，降低了结冰程度。此外，该系统在样本容量方面也表现出了良好的可行性。因此，微流控法是一种很有前途的提高玻璃化冷冻保存效果的方法。(C)2013爱思唯尔有限公司保留所有权利。
Ultra-fast cooling and rewarming are essential to vitrification cryopreservation. In this work the existing cooling/warming approaches are reviewed and then a novel microfluidic system is proposed. The main concerns of the design includes: (a) enhancing the external heat transfer coefficients by utilizing micro-channel array on the chip surface; (b) increasing the sample surface versus its volume by holding the sample inside the system as an ultra-thin film. By alternatively using liquid nitrogen or warm water as its working fluid, the system can be used for both cooling and warming. To assess its application for achieving ultra-fast cooling/warming rates, the magnitudes of external heat transfer coefficients (h) during cooling and warming are theoretically investigated, and then the transient temperature distribution, the cooling/warming rates and the vitrification/devitrification tendencies of sample solution are numerically studied. It is concluded that an ultra-high h (>10(4) W/m(2) K) can be obtained by the microfluidic system in both cooling and warming processes, which efficiently improves the cooling/warming rates and then decreases the degree of ice formation. Moreover, the system demonstrates good feasibility in the capacity of sample volume. Therefore, the microfluidic method should be a promising approach for improving vitrification cryopreservation. (C) 2013 Elsevier Ltd. All rights reserved.