Ginsenoside Rg3 inhibits colon cancer cell migration by suppressing nuclear factor kappa B activity
Ginsenoside Rg3 inhibits colon cancer cell migration by suppressing nuclear factor kappa B activity
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人参皂苷 Rg3 通过抑制核因子 kappa B 活性来抑制结肠癌细胞迁移
DOI：
10.1016/s0254-6272(15)30122-9
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发表时间：
2015-08-15
影响因子：
2.6
通讯作者：
Wang Chaojie
中科院分区：
文献类型：
--
作者：
Song Junmin;Liu Hongxiang;Wang Chaojie
目的：探讨人参皂苷Rg3抑制结肠癌细胞迁移的机制。方法：采用Transwell迁移实验探讨人参皂苷Rg3对SW480细胞迁移的抑制作用。使用电泳迁移率变动分析 (EMSA) 和双荧光素酶报告基因分析来研究 Rg3 对核因子 kappa B (NF-kappa B) 活性的抑制能力。采用Western blotting法测定蛋白水平。结果：200微摩尔人参皂苷Rg3显着抑制SW480细胞迁移（P < 0.05）。 EMSA显示Rg3抑制NF-kappa B的DNA结合能力。双荧光素酶报告基因检测显示Rg3降低NF-kappa B调节的基因转录(P < 0.01)。 Western blots 表明 Rg3 下调 NF-κ B 调节的基质金属蛋白酶 9、环氧合酶-2 和 C-Myc 的表达。 NF-κB抑制剂吡咯烷二硫代氨基甲酸酯增强了Rg3对SW480细胞迁移的抑制作用。结论：人参皂苷Rg3通过抑制NF-κB活性和NF-κB调节基因产物的表达而具有较强的抗肿瘤迁移能力。它可以作为结肠癌患者化疗过程中的良好辅助药物。 (C) 2015 年 JTCM。版权所有。
OBJECTIVE: To study the mechanism of the inhibitory effect of ginsenoside Rg3 on colon cancer cell migration.METHODS: Transwell migration assays were performed to investigate the inhibitory effect of ginsenoside Rg3 on SW480 cell migration. Electrophoretic mobility shift assays (EMSAs) and dual luciferase reporter assays were used to study the suppression capability of Rg3 on nuclear factor kappa B (NF-kappa B) activity. Western blotting was adopted to determine protein levels.RESULTS: Two-hundred micromolar ginsenoside Rg3 significantly inhibited SW480 cell migration (P < 0.05). EMSA showed that Rg3 suppressed the DNA binding ability of NF-kappa B. Dual luciferase reporter assay showed that Rg3 decreased NF-kappa B-regulated gene transcription (P < 0.01). Western blots indicated that Rg3 down-regulated expression of the NF-kappa B-regulated matrix metalloproteinase 9, cyclooxygenase-2 and C-Myc. An NF-kappa B inhibitor, pyrrolidine dithiocarbamate, enhanced the inhibitory effect of Rg3 on SW480 cell migration.CONCLUSION: Ginsenoside Rg3 has a strong antitumor migration capability by suppressing NF-kappa B activity and expression of NF-kappa B-regulated gene products. It could be a good adjuvant for colon cancer patients during the course of chemotherapy. (C) 2015 JTCM. All rights reserved.