Contribution of the Wolffian duct mesenchyme to the formation of the female reproductive tract.
Contribution of the Wolffian duct mesenchyme to the formation of the female reproductive tract.
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沃尔夫管间充质对女性生殖道形成的贡献。
DOI：
10.1093/pnasnexus/pgac182
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Yao,HumphreyHung-Chang
中科院分区：
文献类型：
--
作者：
Zhao,Fei;Grimm,SaraA;Jia,Shua;Yao,HumphreyHung-Chang
雌性生殖道由胚胎的前体苗勒管发育而来。靠近苗勒管的是雄性生殖道的前体，沃尔夫管，它在雌性胚胎的性分化过程中被消除。我们发现沃尔夫氏管的一个组成部分，即间充质，在性别分化后并没有消失。相反，Wolffian管间充质经历了转录组和染色质可及性的变化，从男性道到女性道的身份，并成为女性生殖道中独特的间充质群体，其定位和转录组与来自苗勒管的间充质不同。在离体器官培养中，部分消融沃尔夫管间充质阻碍了胎儿女性生殖道的生长。这些发现揭示了女性生殖道形成的间充质组织的一个新的胎儿起源，并重塑了我们对生殖道性别分化的理解。
The female reproductive tract develops from its embryonic precursor, the Müllerian duct. In close proximity to the Müllerian duct lies the precursor for the male reproductive tract, the Wolffian duct, which is eliminated in the female embryo during sexual differentiation. We discovered that a component of the Wolffian duct, its mesenchyme, is not eliminated after sexual differentiation. Instead, the Wolffian duct mesenchyme underwent changes in transcriptome and chromatin accessibility from male tract to female tract identity, and became a unique mesenchymal population in the female reproductive tract with localization and transcriptome distinct from the mesenchyme derived from the Müllerian duct. Partial ablation of the Wolffian duct mesenchyme stunted the growth of the fetal female reproductive tract in ex vivo organ culture. These findings reveal a new fetal origin of mesenchymal tissues for female reproductive tract formation and reshape our understanding of sexual differentiation of reproductive tracts.