Resistance of Brazilian diamondback moth populations to insecticides
Resistance of Brazilian diamondback moth populations to insecticides
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DOI：
10.1590/s0103-90162011000200004
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发表时间：
2011-04-01
影响因子：
2.6
通讯作者：
Michereff Filho, Miguel
中科院分区：
文献类型：
--
作者：
Oliveira, Alexandre Conte de;Siqueira, Herbert Álvaro Abreu de;Michereff Filho, Miguel
小菜蛾是世界各地十字花科作物上经常出现的害虫。在巴西，通常需要喷洒大量杀虫剂，这可能会导致抗药性快速进化。本研究的目的是评估巴西小菜蛾种群对杀虫剂阿维菌素、溴氰菊酯和多杀菌素的敏感性。叶浸生物测定用于确定接触杀虫剂 48 小时后获得的死亡率数据，并进行 Probit 分析。与参考人群相比，来自巴西伯南布哥州博尼图的人群对阿维菌素的毒性比率最高（20.2 倍）。溴氰菊酯的 LC50 值范围为 85.2 至 360.1 mg L-1，表明相对于该杀虫剂田间剂量率（7.5 mg L-1），种群的高存活率。然而，估计的 LC(50) 毒性比率非常低（从 2.2 倍到 4.2 倍不等）。大多数人群对多杀菌素表现出毒性比，范围为 2.3 至 5.1 倍，而 LC50 和 LC95 值都反映了对多杀菌素现场剂量（120 mg L-1）的高敏感性。只有鲣鱼-PE群体对阿维菌素具有抗性，而所有小菜蛾群体对溴氰菊酯具有抗性，但特别容易受到多杀菌素的影响，因为这些地区不存在选择压力。
Plutella xylostella is a recurring pest on cruciferous crops around the world. In Brazil, it typically requires large number of insecticide sprays, which may lead to fast evolution of resistance. The aim of this study was to assess the susceptibility of Brazilian diamondback moth populations to the insecticides abamectin, deltamethrin, and spinosad. Leaf dip bioassays were used to determine mortality data obtained after 48 h of exposure to insecticides and subjected to Probit analysis. The population from Bonito, state of Pernambuco, Brazil, had the highest toxicity ratio (20.2 - fold) to abamectin compared to the reference population. The LC50 values for deltamethrin ranged from 85.2 to 360.1 mg L-1, demonstrating a high survival of populations in relation to this insecticide field dose rate (7.5 mg L-1). The toxicity ratios of the estimated LC(50)s, however, were very low (varying from 2.2 - to 4.2 - fold). Most populations exhibited toxicity ratios for spinosad, ranging from 2.3 - to 5.1 - fold, while both the LC50 and LC95 values reflected a high susceptibility to the spinosad field dosage (120 mg L-1). Only the Bonito - PE population resisted to abamectin, while all P. xylostella populations were resistant to deltamethrin, but particularly susceptible to spinosad because of the absence of selection pressure with it in these areas.