Identification and expression of the kosena radish (Raphanus sativus cv. Kosena) homologue of the ogura radish CMS-associated gene, orf138
Identification and expression of the kosena radish (Raphanus sativus cv. Kosena) homologue of the ogura radish CMS-associated gene, orf138
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DOI：
10.1023/a:1006198611371
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发表时间：
1999-01-01
影响因子：
5.1
通讯作者：
Imamura, J
中科院分区：
文献类型：
--
作者：
Iwabuchi, M;Koizuka, N;Imamura, J
日本萝卜栽培品种小菜中有一个cms相关基因orf125，该基因序列与小菜cms相关基因orf138同源，除了orf138编码区有两个氨基酸替换和39bp的缺失。在红萝卜中，orf125位点与orfB连接，而在由红萝卜与甘蓝型油菜原生质体融合而成的甘蓝型油菜CMS杂种中，orf125位点不同。在甘蓝型油菜CMS杂交组合orf125的3′侧区发现了一个新的mtDNA序列。orf125在萝卜和甘蓝型油菜CMS杂交中均有表达。其积累与甘蓝型油菜的CMS表型密切相关。甘蓝型油菜的育性恢复伴随着ORF125的减少。
A CMS-associated gene, orf125, present in the Japanese radish cultivar Kosena, has a sequence homologous to that of the ogura CMS-associated gene, orf138, except for two amino acid substitutions and a 39 bp deletion in the orf138 coding region. In Kosena radish, orf125 is linked with orfB, whereas the orf125 locus differs in a Brassica napus CMS cybrid derived from protoplast fusion between Kosena radish and B. napus. A novel mtDNA sequence is present in the 3'-flanking region of orf125 in the B. napus kosena CMS cybrid. The orf125 is expressed both in the radish and the B. napus kosena CMS cybrid. Its accumulation is strongly associated with the CMS phenotype in B. napus. Fertility restoration was accompanied by a decrease in the amount of ORF125 in B. napus.