Environmental pH is a key modulator ofStaphylococcus aureusbiofilm development under predation by the virulent phage phiIPLA-RODI
Environmental pH is a key modulator ofStaphylococcus aureusbiofilm development under predation by the virulent phage phiIPLA-RODI
复制标题
DOI：
10.1038/s41396-020-00778-w
复制
发表时间：
2020-09-22
期刊：
影响因子：
11
通讯作者：
Rodriguez, Ana
中科院分区：
文献类型：
--
作者：
Fernandez, Lucia;Gutierrez, Diana;Rodriguez, Ana
先前的工作表明，在一些金黄色葡萄球菌菌株中，低浓度的毒性噬菌体vB_SauM_phiIPLA-RODI（phiIPLA-RODI）促进了富含DNA的生物膜的形成，其细胞与未感染的对照相比表现出显著的转录差异。本研究旨在剖析导致这些变化的事件序列。在整个生物膜发育过程中噬菌体繁殖的分析显示，噬菌体颗粒的数量稳定增加到一定程度，然后下降。这种部分噬菌体失活似乎是由于细菌的葡萄糖发酵导致的培养基酸化的结果。在生物膜发育过程中噬菌体-宿主动力学的计算机模拟显示了pH演变中的微小差异如何影响噬菌体感染的结果。一个酸性的pH值，连同成功的噬菌体繁殖，也是必要的，以观察噬菌体相关的生物膜结构和细菌种群的转录谱的变化。总之，这项研究表明，噬菌体和宿主之间的动态如何通过环境线索紧密协调，即使在复杂的生物膜人口的背景下。
Previous work had shown that, in someStaphylococcus aureusstrains, low concentrations of the virulent phage vB_SauM_phiIPLA-RODI (phiIPLA-RODI) promoted the formation of DNA-rich biofilms, whose cells exhibited significant transcriptional differences compared to an uninfected control. This study aimed to dissect the sequence of events leading to these changes. Analysis of phage propagation throughout biofilm development revealed that the number of phage particles increased steadily up to a certain point and then declined. This partial phage inactivation seemed to be a consequence of medium acidification due to glucose fermentation by the bacterium. Computer simulation of phage-host dynamics during biofilm development showed how even small differences in pH evolution can affect the outcome of phage infection. An acidic pH, together with successful phage propagation, was also necessary to observe the phage-associated changes in biofilm architecture and in the transcriptional profile of the bacterial population. Altogether, this study shows how the dynamics between phage and host can be tightly coordinated through an environmental cue, even in the context of a complex biofilm population.