INTRAVENOUS HYPERALIMENTATION WITH HIGH ARGININE LEVELS IMPROVES WOUND-HEALING AND IMMUNE FUNCTION
INTRAVENOUS HYPERALIMENTATION WITH HIGH ARGININE LEVELS IMPROVES WOUND-HEALING AND IMMUNE FUNCTION
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DOI：
10.1016/0022-4804(85)90045-9
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发表时间：
1985-01-01
影响因子：
2.2
通讯作者：
EFRON, G
中科院分区：
文献类型：
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作者：
BARBUL, A;FISHEL, RS;EFRON, G
本研究的目的是评价静脉高营养(IVH)治疗中精氨酸水平升高对创面愈合和胸腺免疫功能的影响。SD大鼠275~325g，分别行颈内静脉置管、背部皮肤损伤7 cm、皮下植入聚乙烯醇海绵、不锈钢缝合创面。24小时后，大鼠以0.8~1ml/100g体重/小时的速度开始IVH。所有IVH溶液都含有20%的葡萄糖、充足的矿物质和维生素，以及两种不同的氨基酸混合物：(A)Fre III(4.05g Arg/L)(n=13)；(B)实验(7.50g Arg/L)(n=11)。溶液是等氮的，并且含有类似数量的必需氨基酸。IVH后7天，两组的体重增长无差异，但A组的累积氮平衡优于A组；B组的创面愈合情况以新鲜创面条断裂强度、固定断裂强度和修复胶原沉积量(以植入的海绵中的羟脯氨酸含量评估)来评估。B组动物的胸腺重量、胸腺淋巴细胞总数和胸腺淋巴细胞对PHA和ConA的促有丝分裂反应性也有改善。实验表明，高水平的IVH液中的精氨酸水平提高了创伤愈合和胸腺免疫功能。
The purpose of this study was to evaluate the effect of increased arginine levels in intravenous hyperalimentation (IVH) therapy on wound healing and thymic immune function. Groups of SD rats, 275–325 g, underwent placement of internal jugular catheter, 7-cm dorsal skin wounding, insertion of polyvinyl alcohol sponges subcutaneously, and closure of wounds with stainless-steel sutures. Twenty-four hours later, rats were started on IVH at a rate of 0.8–1 ml/100 g body wt/hr. All IVH solutions contained 20% dextrose, adequate amounts of minerals and vitamins, and two different amino acid mixtures: (A) Fre III (4.05 g ARG/liter) (n= 13); (B) experimental (7.50 g ARG/liter) (n= 11). Solutions were isonitrogenous, and contained similar amounts of essential amino acids. After 7 days of IVH, weight gain did not differ between the two groups; however, cumulative N balance was superior in group A. Wound healing was improved in group B as assessed by fresh wound strip breaking strength, fixed breaking strength, and the amount of reparative collagen deposition as assessed by the hydroxyproline content of the implanted sponges. Group B animals also had improved thymic function as assessed by thymic weight, the total number of thymic lymphocytes/gland and mitogenic reactivity of thymic lymphocytes to PHA and Con A. The experiments indicate that high arginine levels in IVH solutions improve wound healing and thymic immune function following injury.