Identification of trait-improving quantitative trait loci alleles from a wild rice relative, Oryza rufipogon.
Identification of trait-improving quantitative trait loci alleles from a wild rice relative, Oryza rufipogon.
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发表时间：
1998-10
期刊：
影响因子：
3.3
通讯作者：
Jinhua Xiao;Jiming Li;S. Grandillo;S. Ahn;L. Yuan;S. Tanksley;S. McCouch
中科院分区：
文献类型：
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作者：
Jinhua Xiao;Jiming Li;S. Grandillo;S. Ahn;L. Yuan;S. Tanksley;S. McCouch
野生物种被视为遗传变异的独特来源，但它们很少用于数量性状的遗传改良。为了从外来种中鉴定改良性状的数量性状基因座（QTL）等位基因，在遗传多样性研究的基础上选择了栽培稻的近缘种普通野生稻（Oryza rufipogon）。利用种间BC_2测交群体（V20 A/O. V20 B/V20 B/Ce 64）的12个重要数量性状进行了评价。手术普通芒属的加入对于所有12个性状都是表型较差的。然而，超亲分离，优于原来的精英杂交品种，V20 A/Ce 64，观察到的所有性状检查。利用一组122个RFLP和微卫星标记进行QTL定位。共检测到68个显著QTL，其中35个（51%）具有来自表型劣势O.普通猪笼草亲本这些有益的QTL等位基因中有19个（54%）对其他性状没有有害影响。O.在第1和第2染色体上的两个QTL上的rufipogon等位基因分别与单株籽粒产量增加18%和17%相关，而不延迟成熟或增加株高。这一发现表明，分子图谱和标记的创新使用可以改变遗传学家利用野生和外来种质的方式。
Wild species are valued as a unique source of genetic variation, but they have rarely been used for the genetic improvement of quantitative traits. To identify trait-improving quantitative trait loci (QTL) alleles from exotic species, an accession of Oryza rufipogon, a relative of cultivated rice, was chosen on the basis of a genetic diversity study. An interspecific BC2 testcross population (V20A/O. rufipogon//V20B///V20B////Ce64) consisting of 300 families was evaluated for 12 agronomically important quantitative traits. The O. rufipogon accession was phenotypically inferior for all 12 traits. However, transgressive segregants that outperformed the original elite hybrid variety, V20A/Ce64, were observed for all traits examined. A set of 122 RFLP and microsatellite markers was used to identify QTL. A total of 68 significant QTL were identified, and of these, 35 (51%) had beneficial alleles derived from the phenotypically inferior O. rufipogon parent. Nineteen (54%) of these beneficial QTL alleles were free of deleterious effects on other characters. O. rufipogon alleles at two QTL on chromosomes 1 and 2 were associated with an 18 and 17% increase in grain yield per plant, respectively, without delaying maturity or increasing plant height. This discovery suggests that the innovative use of molecular maps and markers can alter the way geneticists utilize wild and exotic germplasm.