Schisandrin B ameliorated chondrocytes inflammation and osteoarthritis via suppression of NF-κB and MAPK signal pathways.
Schisandrin B ameliorated chondrocytes inflammation and osteoarthritis via suppression of NF-κB and MAPK signal pathways.
复制标题
五味子 B 通过抑制 NF-κB 和 MAPK 信号通路改善软骨细胞炎症和骨关节炎。
DOI：
10.2147/dddt.s162014
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Wu L
中科院分区：
文献类型：
--
作者：
Ran J;Ma C;Xu K;Xu L;He Y;Moqbel SAA;Hu P;Jiang L;Chen W;Bao J;Xiong Y;Wu L
骨关节炎（Osteoarthritis，OA）是老年人群中最常见的关节疾病，IL-1β等炎症介质在OA的发生发展中起着重要作用。北五味子醇甲B是北五味子的主要活性成分，具有抗氧化和抗氧化作用。本研究通过体内外实验，探讨了白藜芦醇B对OA的保护作用及其机制。结果表明，五味子乙素B降低了IL-1β诱导的基质金属蛋白酶3（MMP 3）、MMP 13、IL-6和诱导型一氧化氮合酶（iNOS）的上调，并增加了IL-1β诱导的II型胶原、聚集蛋白聚糖和sox 9的下调。五味子乙素B可显著降低IL-1β诱导的大鼠软骨细胞p65磷酸化和p65核转位。丝裂原活化蛋白激酶（MAPK）的激活也被抑制，如通过减少p38，细胞外信号调节激酶（Erk），和c-Jun氨基末端激酶（JNK）磷酸化证明的，野菊花苷B。此外，五味子乙素B可预防大鼠OA模型中的软骨退化，Mankin评分显著低于对照组。我们的研究表明，五味子乙素B通过抑制核因子-κB（NF-κB）和MAPK信号通路来改善软骨细胞炎症和OA，表明其在OA治疗中的治疗潜力。
Osteoarthritis (OA) is the most prevalent joint disorder in the elderly population, and inflammatory mediators like IL-1β were thought to play central roles in its development. Schisandrin B, the main active component derived from Schisandra chinensis, exhibited anti-oxidative and antiinflammatory properties. In the present study, the protective effect and the underlying mechanism of Schisan-drin B on OA was investigated in vivo and in vitro. The results showed that Schisandrin B decreased IL-1β-induced upregulation of matrix metalloproteinase 3 (MMP3), MMP13, IL-6, and inducible nitric oxide synthase (iNOS) and increased IL-1β-induced downregulation of collagen II, aggrecan, and sox9 as well. Schisandrin B significantly decreased IL-1β-induced p65 phosphorylation and nuclear translocation of p65 in rat chondrocytes. Mitogen-activated protein kinase (MAPK) activation was also inhibited by Schisandrin B, as evidenced by the reduction of p38, extracellular signal-regulated kinase (Erk), and c-Jun amino-terminal kinase (Jnk) phosphorylation. In addition, Schisandrin B prevented cartilage degeneration in rat OA model with significantly lower Mankin’s score than the control group. Our study demonstrated that Schisandrin B ameliorated chondrocytes inflammation and OA via suppression of nuclear factor-κB (NF-κB) and MAPK signal pathways, indicating a therapeutic potential in OA treatment.