Semen analysis performed by different laboratory teams: an intervariation study
Semen analysis performed by different laboratory teams: an intervariation study
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DOI：
10.1046/j.1365-2605.1997.00052.x
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发表时间：
1997-08-01
影响因子：
--
通讯作者：
Skakkebaek, NE
中科院分区：
文献类型：
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作者：
Jorgensen, N;Auger, J;Skakkebaek, NE
最近的一些研究表明，精子浓度在过去50年中有所下降。然而，实验室之间的比较表明，地理差异似乎存在，而且任何下降可能不是全球性的。关于比较不同实验室的结果的一个批评意见是，发现的一些差异可能反映了不同实验室缺乏标准化的方法。四个小组，每个小组由一名医生和一名技术人员组成，他们来自最近发表了精液质量数据的小组，他们开会评估他们实验室之间精液分析的差异。对26例男性新鲜精液进行了分析。这些小组根据实验室的正常做法，使用自己的设备分析了样本。通过随机效应模型估计实验室之间的变异。对于精子浓度和精液量评估，检测到实验室之间的显着一致性，与非常可观的个体间差异相反。对于精子活力和形态学评估，实验室间一致性较差。总之，精子活力和形态特征的评价需要进一步标准化，以获得来自不同实验室的可比数据。然而，当使用相似的方法时，精液量和精子浓度是可以在实验室之间进行可靠比较的特征。
Some recent studies have indicated that sperm concentration has decreased during the last 50 years. However, comparisons between laboratories have revealed that geographical differences seem to exist and that any decrease may not be global. One point of criticism concerning comparison of results from different laboratories has been that some of the discrepancies detected could reflect the lack of standardized methods used in the different laboratories. Four teams, each consisting of one physician and one technician from groups which have recently published data on semen quality, met in order to evaluate the variability between their laboratories on semen analysis. Twenty-six fresh semen samples from unselected men were analysed. The groups analysed the samples according to the normal practice in their laboratories, using their own equipment. The variation between laboratories was estimated through a random effects model. For sperm concentration and semen volume assessment a remarkable consistency between laboratories was detected, in contrast to the very considerable interindividual variation. For sperm motility and morphology assessments interlaboratory consistency was much poorer. In conclusion, evaluation of sperm motility and morphology characteristics requires further standardization in order to achieve comparable data from different laboratories. However, semen volume and sperm concentration are characteristics which can be compared reliably between laboratories, when similar methodologies are used.