The ER chaperone and signaling regulator GRP78/BiP as a monitor of endoplasmic reticulum stress
The ER chaperone and signaling regulator GRP78/BiP as a monitor of endoplasmic reticulum stress
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DOI：
10.1016/j.ymeth.2004.10.010
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发表时间：
2005-04-01
期刊：
影响因子：
4.8
通讯作者：
Lee, AS
中科院分区：
文献类型：
--
作者：
Lee, AS
内质网应激以及未折叠蛋白反应在健康和疾病中的多重影响凸显了在各种实验或生理环境下确定其发生的便捷监测系统的重要性。大量研究证实，葡萄糖调节蛋白78（GRP78）的诱导是内质网应激的一个标志物。GRP78，也被称为免疫球蛋白重链结合蛋白（BiP），是内质网应激的核心调节因子，因为它作为一种主要的内质网伴侣蛋白具有抗凋亡特性，并且能够通过结合 - 释放机制控制跨膜内质网应激传感器（肌醇需求酶1（IRE1）、蛋白激酶R样内质网激酶（PERK）和活化转录因子6（ATF6））的激活。在接下来的报告中，我们介绍了几种测量GRP78诱导的方法。这可以通过测量Grp78启动子活性，或者通过测量Grp78转录本水平或GRP78蛋白水平来实现。这些技术可应用于组织培养细胞以及组织和器官。（c）2004爱思唯尔公司。保留所有权利。
The multiple implications of ER stress and the unfolded protein response in health and disease highlight the importance of identifying convenient monitoring systems for its onset under various experimental or physiological settings. A large volume of studies establish that induction of GRP78 is a marker for ER stress. GRP78, also referred to as BiP, is a central regulator for ER stress due to its role as a major ER chaperone with anti-apoptotic properties as well as its ability to control the activation of transmembrane ER stress sensors (IRE1, PERK, and ATF6) through a binding-release mechanism. In the following report, we present several methods to measure GRP78 induction. This can be achieved by measuring the Grp78 promoter activity or by measuring the level of Grp78 transcripts or GRP78 protein. These techniques can be applied to tissue culture cells as well as tissues and organs. (c) 2004 Elsevier Inc. All rights reserved.