Identification of plexin family molecules in mice
Identification of plexin family molecules in mice
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DOI：
10.1006/bbrc.1996.1367
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发表时间：
1996-09-13
影响因子：
3.1
通讯作者：
Fujisawa, H
中科院分区：
文献类型：
--
作者：
Kameyama, T;Murakami, Y;Fujisawa, H
通过筛选E17.5小鼠脑cDNA文库，我们分离到两个编码新的丛蛋白样蛋白的cDNA。测序结果表明，这两种蛋白质是1型膜蛋白，在氨基酸水平上与小鼠丛蛋白1具有超过60%的同一性。此外，这两种蛋白质的推定的细胞外片段具有三个重复的富含半胱氨酸的结构域，这是丛蛋白的共同基序。因此，我们将这两种蛋白质命名为小鼠丛蛋白2和小鼠丛蛋白3。我们获得了缺失部分蛋白编码区的小鼠丛蛋白3 cDNA克隆。此外，北方印迹分析显示小鼠丛蛋白2 mRNA的分子异质性。这些发现表明，在小鼠中，丛蛋白包括一个分子家族（丛蛋白家族）。(C)出版社：Academic Press，Inc.
By screening of E17.5 mouse brain cDNA libraries, we isolated two cDNAs encoding new plexin-like proteins. Sequencing revealed that these two proteins were type 1 membrane proteins which showed over 60% identity at the amino acid level to mouse plexin 1. Moreover, putative extracellular segments of these two proteins had three repeats of a cysteine-rich domain which is a common motif for plexin proteins. Thus, we named these two proteins mouse plexin 2 and mouse plexin 3. We obtained mouse plexin 3 cDNA clones in which a part of protein-coding region was deleted. Also, Northern blot analysis showed molecular heterogeneity in mouse plexin 2 mRNAs. These findings indicate that, in the mouse, plexins comprise a molecular family (the plexin family). (C) 1996 Academic Press, Inc.