Modulation of firing activity by endogenous GABAA receptors in the globus pallidus of MPTP-treated parkinsonian mice
Modulation of firing activity by endogenous GABAA receptors in the globus pallidus of MPTP-treated parkinsonian mice
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DOI：
10.1007/s12264-013-1351-x
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发表时间：
2013-12-01
影响因子：
5.6
通讯作者：
Chen, Lei
中科院分区：
文献类型：
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作者：
Chen, Xin-Yi;Xue, Yan;Chen, Lei
啮齿类动物的苍白球相当于灵长类动物的苍白球外段，在运动调节中起着重要作用。以往的研究表明，在苍白球中存在丰富的γ -氨基丁酸（GABA）能神经支配和GABA(A)受体。在本研究中，我们利用多管电极在体内细胞外记录的方法，研究了内源性GABA(A)受体对正常和mptp处理小鼠苍白球神经元自发放电活动的影响。我们发现，在正常小鼠中，0.1 mmol/L gabazine（一种特异性GABA(a)受体拮抗剂）的压力射能使苍白球神经元的自发放电率增加27.6±5.6%。此外，在MPTP小鼠中（MPTP治疗后14天），0.1 mmol/L gabazine使放电率提高了51.0±7.9%，显著高于正常小鼠。这些结果提示内源性GABA(A)受体调节苍白球神经元的活性。目前的发现可能为研究GABA(a)受体在帕金森病中的潜在作用提供了理论依据。
The globus pallidus in rodents, equivalent to the external segment of the globus pallidus in primates, plays an important role in movement regulation. Previous studies have shown abundant gamma-aminobutyric acid (GABA)ergic innervation and GABA(A) receptors in the globus pallidus. In this study, we investigated the effects of endogenous GABA(A) receptors on the spontaneous firing activity of pallidal neurons in both normal and MPTP-treated mice using multi-barrel electrodes extracellular recordings in vivo. We found that in normal mice, pressure ejection of 0.1 mmol/L gabazine, a specific GABA(A) receptor antagonist, increased the spontaneous firing rate of globus pallidus neurons by 27.6 +/- 5.6%. Furthermore, in MPTP mice (14 days after MPTP treatment), 0.1 mmol/L gabazine increased the firing rates by 51.0 +/- 7.9%, significantly greater than in normal mice. These results suggest that endogenous GABA(A) receptors modulate the activity of globus pallidus neurons. The present findings may provide a rationale for investigations into the potential role of GABA(A) receptors in Parkinson's disease.