Ontogeny of immunity in amphibians: Changes in antibody repertoires and appearance of adult major histocompatibility antigens in Xenopus
Ontogeny of immunity in amphibians: Changes in antibody repertoires and appearance of adult major histocompatibility antigens in Xenopus
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两栖动物免疫的个体发育：非洲爪蟾抗体库的变化和成年主要组织相容性抗原的出现
DOI：
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发表时间：
1979
影响因子：
5.4
通讯作者：
C. Bernard
中科院分区：
文献类型：
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作者：
L. Du Pasquier;B. Blomberg;C. Bernard
可通过等电聚焦（IEF）分析低分子量类型的爪蟾幼虫抗2，4-二硝基苯基抗体。在基因相同的克隆动物中，蝌蚪产生的抗体的IEF谱型与大多数个体相同。同一克隆的成虫也产生相同谱型的抗体，但成虫的模式可能与幼虫的模式非常不同，尽管两者的反应是异质的。如果成虫在幼虫生活期间被激发并在变态后被增强，则可以发现在初级成虫反应中看不到的幼虫谱型。蝌蚪（最多12条抗体IEF条带）的反应异质性略低于成体（最多20条抗体IEF条带）。在库的变化发生在变态的时候出现的两个主要组织相容性复合体抗原。一种是淋巴细胞抗原，在变态结束前10 - 15天出现，另一种是红细胞（可能也在淋巴细胞上），在变态结束后1.5个月出现，通过免疫荧光分析确定。在同一时期，同系混合白细胞反应从幼虫抗成虫反应转变为成虫抗幼虫反应。
Larval Xenopus anti‐2, 4‐dinitrophenyl antibodies of the low‐molecular weight type can be analyzed by isoelectric focusing (IEF). Within a clone of genetically identical animals, tadpoles make antibodies whose IEF spectrotypes are shared by most of the individuals. Adults of the same clone also make antibodies of identical spectrotype, but the adult pattern can be very different from the larval one, although both responses are heterogeneous. The larval spectrotypes that one cannot see in a primary adult response can be found if the adult has been primed during larval life and boosted after metamorphosis. The heterogeneity of the response is somewhat lower in tadpoles (up to 12 antibody IEF bands) than in adults (up to 20 antibody IEF bands). The change in the repertoire occurs during metamorphosis at the time of the appearance of two major histocompatibility complex antigens. One, a lymphocyte antigen, appears 10‐15 days before the end of metamorphosis, the other, present on red cells (and presumably also on lymphocytes), appears 1.5 month after the end of metamorphosis, as determined by immunofluorescence analysis. During the same period, the syngeneic mixed leukocyte reaction switches from a larval anti‐adult to an adult anti‐larval reaction.