POLYSOMES, MESSENGER-RNA, AND GROWTH IN SOYBEAN STEMS DURING DEVELOPMENT AND WATER DEFICIT
POLYSOMES, MESSENGER-RNA, AND GROWTH IN SOYBEAN STEMS DURING DEVELOPMENT AND WATER DEFICIT
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DOI：
10.1104/pp.86.3.725
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发表时间：
1988-03-01
期刊：
影响因子：
7.4
通讯作者：
BOYER, JS
中科院分区：
文献类型：
--
作者：
MASON, HS;MULLET, JE;BOYER, JS
研究了不同区域黑豆（Glycine max）多体状态和多体mRNA的居群。(L。包含分裂的、伸长的或成熟的（不生长的）细胞的茎。多体含量呈现分化组织最高、成熟组织最低的发育梯度。将幼苗移栽到含水量（水势）较低的蛭石上数小时后。W = -0.29 megapascal)，茎生长速率下降至水分充足对照的30%，多聚体含量下降最多的是分裂组织和伸长组织。24 ~ 36 h后，茎生长和多聚体含量逐渐恢复。在二维凝胶上检测了来自分裂、伸长或成熟组织的多体mRNA的体外翻译产物。在水分充足的对照中，每个茎区都富含一小部分多体mRNA群体，这可能是由于发育调节的基因表达。将植物暴露在低psi环境中。24小时w诱导了伸长和成熟组织中少量多体mrna相对丰度的变化，但在分裂组织中没有。24到72小时后。w，在伸长组织中，多体mRNA群体的变化是相反的。这些数据表明，低水势条件下茎生长的变化与伸长组织中多体状态和多体mRNA的变化有关。
The polysome status and populations of polysomal mRNA were examined in different regions of dark-grown soybean (Glycine max. [L.] Merr.) stems that contained either dividing, elongating, or mature (non-growing) cells. There was a developmental gradient of polysome content in which the dividing tissue had the highest levels and the mature tissue the lowest. A few hours after transplanting the seedlings to vermiculite having low water content (water potential .psi.w = -0.29 megapascals), stem growth rate decreased to 30% of well-watered controls and the polysome content decreased most in the dividing and elongating tissues. After 24 to 36 hours, stem growth and polysome content recovered gradually. In vitro translation products of polysomal mRNA from dividing, elongating or mature tissue were examined on two-dimensional gels. In well-watered controls, each of the stem regions was enriched in a small subset of the polysomal mRNA population, probably because of developmentally regulated gene expression. Exposing plants to low .psi.w for 24 hours induced a change in the relative abundance of a small number of polysomal mRNAs in the elongating and mature tissues, but not in the dividing tissue. After 24 to 72 hours at low .psi.w, the changes in polysomal mRNA population were reversed in the elongating tissue. The data indicate that changes in stem growth at low water potential are associated with changes in polysome status and polysomal mRNA in the elongating tissue.