LYMPHOCYTE-T POPULATIONS IN FETAL ALCOHOL SYNDROME
LYMPHOCYTE-T POPULATIONS IN FETAL ALCOHOL SYNDROME
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DOI：
10.1111/j.1530-0277.1989.tb00362.x
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发表时间：
1989-08-01
期刊：
影响因子：
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通讯作者：
EWALD, SJ
中科院分区：
文献类型：
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作者：
EWALD, SJ
有报道称胎儿酒精综合征（FAS）与免疫缺陷或DiGeorge综合征有关。为了研究产前酒精暴露对免疫系统的影响，我们使用FAS小鼠模型，其中C57 BL/6 J雌性小鼠从妊娠日（g.d.）1到18。在暴露于乙醇的18天胎儿中，胸腺细胞数量明显减少。通过流式细胞术比较来自25%EDC饮食组和来自成对喂养和自由采食对照饮食组的胎儿胸腺细胞的T细胞分化抗原表达。与对照组相比，暴露于酒精的胎儿中L3 T4和Lyt-2阳性胸腺细胞的比例显著降低;然而，Thy-1阳性细胞的数量在任何组之间均无差异。出生6天的新生儿产前暴露于g.d.的乙醇。在几乎所有情况下，1至13的胸腺和脾脏T细胞群与对照组相似，表明大多数动物的T细胞数量“追赶”。脾T细胞功能，通过对刀豆球蛋白A（Con A）或Con A加T细胞生长因子的反应进行评估，在乙醇暴露6天的幼仔中有所抑制。
There are reports that fetal alcohol syndrome (FAS) is associated with immune deficiency or DiGeorge syndrome. To investigate the effect of prenatal alcohol exposure on the immune system, we used a mouse model of FAS in which C57BL/6J female mice were fed a complete liquid diet containing 25% ethanol-derived calories (EDC) from gestational day (g.d.) 1 to 18. Thymus cell numbers were markedly reduced in 18-day fetuses exposed to ethanol. Thymocytes from fetuses from the 25% EDC diet group and from pair-fed and ad-libitum control diet groups were compared by flow cytometry for expression of T cell differentiation antigens. The proportions of L3T4- and Lyt-2 positive thymus cells were significantly reduced in alcohol-exposed fetuses compared to controls; however, the number of Thy-1-positive cells did not differ among any of the groups. Six-day old neonates exposed prenatally to ethanol from g.d. 1 to 13 had thymus and spleen T cell populations similar to those of controls in almost all cases, indicating a "catch-up" of T cell numbers in most animals. Spleen T cell function, assessed by response to Concanavalin A (Con A), or Con A plus T cell growth factors, was somewhat depressed in ethanol-exposed 6-day pups.