A retrofitted neural recording system with a novel stimulation IC to monitor early neural responses from a stimulating electrode.
A retrofitted neural recording system with a novel stimulation IC to monitor early neural responses from a stimulating electrode.
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改造后的神经记录系统配有新型刺激 IC，可监测刺激电极的早期神经反应。
DOI：
10.1016/j.jneumeth.2008.11.017
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发表时间：
2009
影响因子：
3
通讯作者：
DeWeerth,StephenP
中科院分区：
文献类型：
--
作者：
Nam,Yoonkey;Brown,EdgarA;Ross,JamesD;Blum,RichardA;Wheeler,BruceC;DeWeerth,StephenP
细胞外电刺激越来越多地用于体外神经实验，包括脑切片和培养细胞。尽管期望直接从刺激电极记录，但是相对高的刺激水平使得在刺激之后立即记录非常困难。我们已经证明，通过包括主动电极放电功能的刺激系统（模拟IC），这是可行的。在这里，我们将新的IC搭载到现有的记录放大器系统上，使其能够在刺激后3ms直接从刺激通道记录神经反应。我们使用改装的记录系统，刺激和记录分离的海马神经元在文化。这种改造现有系统的新策略对于有兴趣在不替换现有记录系统的情况下为细胞外记录添加新功能的仪器设计者来说是一种简单但有吸引力的方法。
Extracellular electrical stimulation is increasingly used for in vitro neural experimentation, including brain slices and cultured cells. Although it is desirable to record directly from the stimulating electrode, relatively high stimulation levels make it extremely difficult to record immediately after the stimulation. We have shown that this is feasible by a stimulation system (analog IC) that includes the feature of active electrode discharge. Here, we piggybacked the new IC onto an existing recording amplifier system, making it possible to record neural responses directly from the stimulating channel as early as 3ms after the stimulation. We used the retrofitted recording system to stimulate and record from dissociated hippocampal neurons in culture. This new strategy of retrofitting an existing system is a simple but attractive approach for instrumentation designers interested in adding a new feature for extracellular recording without replacing already existing recording systems.