Adsorption and release properties of growth factors from biodegradable implants
Adsorption and release properties of growth factors from biodegradable implants
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DOI：
10.1002/jbm.1258
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发表时间：
2002-03-05
期刊：
影响因子：
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通讯作者：
Günther, KP
中科院分区：
文献类型：
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作者：
Ziegler, J;Mayr-Wohlfart, U;Günther, KP
本研究旨在研究生长因子在生物可降解植入物中的吸附行为及其体外释放特性。我们研究了在各种支架上施用的生长因子的稳定性。我们使用多孔磷酸三钙陶瓷（α-TCP）、中和玻璃陶瓷（GB 9 N）、复合物（聚乳酸/-乙交酯/GB 9 N）和溶剂脱水人骨作为载体。块状支架（尺寸：7 × 7 × 10 mm）中加载5 μ g溶于150穆尔PBS中的骨形态发生蛋白（rxBMP-4）、碱性成纤维细胞生长因子（rh-bFGF）或血管内皮生长因子（rh-VEGF）。用碘125（I-125）标记生长因子，通过计数样品的总I-125活性来检测生长因子的吸附和释放量。我们观察到，这些生长因子的吸附似乎取决于两个不同的参数：第一个是测试材料的性质，第二个是生长因子本身。生长因子从可生物降解的植入物中的释放动力学可以描述为两阶段过程-在最初几个小时内通过洗脱未吸附的蛋白质而非常快速地释放，随后是特定的释放，这取决于所用材料和生长因子的化学/物理相互作用。在SDS-PAGE上分析洗脱的蛋白质，rh-VEGF被降解成大小约为15 kDa的较小片段，而rxBMP-4和rh-bFGF在超过3天后显示出完全降解成小于3 kDa的片段。虽然这项体外研究表明，可生物降解的植入物可能会成功地用作载体的成骨生长因子，不同的释放动力学，以及其分子结构的改变，包括生物活性的损失，应予以考虑。(C)John Wiley & Sons，Inc. J Biomed Mater Res 59：422-428，2002.
The present investigation was performed to study the adsorption behavior of growth factors and their release characteristics from biodegradable implants in an in vitro study. We investigated the stability of growth factors administered on various scaffolds, We used porous tricalcium phosphate ceramics (alpha -TCP), a neutralized glass-ceramics (GB9N), a composite (polylactid/-glycolid/GB9N), and solvent dehydrated human bone as carriers. Block shaped scaffolds (sized: 7 x 7 x 10 mm) were loaded with 5 mug of either bone morphogenetic protein (rxBMP-4), basic fibroblast growth factor (rh-bFGF), or vascular endothelial growth factor (rh-VEGF) solved in 150 muL PBS. The growth factors were labeled with Iodine125 (I-125) for detecting the adsorbed and released amount of growth factors by counting the samples for total I-125 activity. We observed that the adsorption of these growth factors seems to depend on two different parameters: first on the nature of the tested material, and second on the growth factors on their own. The release kinetics of the growth factors from the biodegradable implants can be described as a two phase process-a very rapid release during the first hours by an elution of not adsorbed protein, followed by a specific release, which depends upon the chemical/physical interaction of the material and the growth factor used. Analyzing the eluted proteins on SDS-PAGEs rh-VEGF was degraded into a smaller fragment with a size of around 15 kDa, while rxBMP-4 and rh-bFGF showed a complete degradation into fragments smaller than 3 kDa after more than 3 days. Although this in vitro study suggests that biodegradable implants might be successfully used as carriers for osteogenic growth factors, the different release kinetics as well as the alteration of their molecular structure including loss of biological activity should be considered. (C) 2001 John Wiley & Sons, Inc. J Biomed Mater Res 59: 422-428, 2002.