Beetle immunity:: Identification of immune-inducible genes from the model insect Tribolium castaneum
Beetle immunity:: Identification of immune-inducible genes from the model insect Tribolium castaneum
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DOI：
10.1016/j.dci.2007.09.005
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发表时间：
2008-01-01
影响因子：
2.9
通讯作者：
Vilcinskas, Andreas
中科院分区：
文献类型：
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作者：
Altincicek, Boran;Knorr, Eileen;Vilcinskas, Andreas
赤拟谷盗（Tribolium castaneum）是一种已建立的遗传学上易于处理的用于进化和发育研究的模式昆虫。因此，它也可能代表一个有价值的模型，比较分析昆虫免疫。在这里，我们使用的抑制消减杂交方法，以确定拟谷盗基因的转录诱导注射粗脂多糖（LPS）。确定的基因编码的蛋白质与已知介导感染感测（例如Toll和PGRP）或代表潜在抗菌效应物（例如铁蛋白、c型溶菌酶、丝氨酸蛋白酶抑制剂和防御素）的其他昆虫的对应物具有序列相似性。特别重要的是鉴定了奇异果甜蛋白样肽，代表最初从植物中报道的古老抗真菌肽，其在许多其他昆虫如果蝇、按蚊和蜜蜂的基因组中不存在。我们在细菌中产生了重组奇异果甜蛋白-1，我们发现它代表了一种抗拟谷盗属丝状真菌的抗菌肽。此外，脓毒性损伤诱导赤拟谷盗属中参与应激适应（例如热休克蛋白）或杀虫剂抗性（例如细胞色素P450）的基因的表达，这表明免疫和应激反应之间可能存在串扰。(c)2007爱思唯尔有限公司保留所有权利。
The red flour beetle, Tribolium castaneum, is an established genetically tractable model insect for evolutionary and developmental studies. Therefore, it may also represent a valuable model for comparative analysis of insect immunity. Here, we used the suppression subtractive hybridization method to identify Tribolium genes that are transcriptionally induced in response to injection of crude lipopolysaccharide (LPS). Determined genes encode proteins that share sequence similarities with counterparts from other insects known to mediate sensing of infection (e.g. Toll and PGRP) or to represent potential antimicrobial effectors (e.g. ferritin, c-type lysozyme, serine proteinase inhibitors, and defensins). Especially significant is the identification of thaumatin-like peptides, representing ancient antifungal peptides originally reported from plants, that are absent from the genomes of many other insects such as Drosophila, Anopheles, and Apis. We produced recombinant thaumatin-1 in bacteria and we found that it represents an antimicrobial peptide against filamentous fungi in Tribolium. Additionally, septic injury induces expression of genes involved in stress adaptation (e.g. heat-shock proteins) or insecticide resistance (e.g. cytochrome P450s) in Tribolium, suggesting that there may be crosstalk between the immune and stress responses. (c) 2007 Elsevier Ltd. All rights reserved.