Three naturally occurring host defense peptides protect largemouth bass (Micropterus salmoides) against bacterial infections
Three naturally occurring host defense peptides protect largemouth bass (Micropterus salmoides) against bacterial infections
复制标题
三种天然存在的宿主防御肽可保护大口黑鲈（Micropterus salmoides）免受细菌感染
DOI：
10.1016/j.aquaculture.2021.737383
复制
发表时间：
2021-08-25
期刊：
影响因子：
4.5
通讯作者：
Wang, Yipeng
中科院分区：
文献类型：
--
作者：
Ouyang, Jianhong;Zhu, Yiyun;Wang, Yipeng
由耐药细菌引起的传染病是水产养殖业面临的重大挑战。迫切需要创新的抗感染药物和/或治疗策略。宿主防御肽（HDPs）是一种天然存在的多肽，被认为是传统抗生素的有前途的替代品。然而，它们作为抗菌剂在水产养殖中的应用还没有得到很好的研究。在本研究中，三个先前表征的HDPs（CATHPb 1，Cm-CATH 2，和HC-CATH）的选择和它们的保护能力对大口黑鲈（Micropterus salmoides）对细菌感染进行了测定。3种HDP对水生病原菌具有较强的广谱抗菌活性，MIC值为2.34-18.75 μ g/ml。它们通过诱导细胞膜透化和细胞破裂在30分钟内迅速杀死细菌。它们还表现出很强的抑制细菌生物膜形成和消除预先形成的生物膜的能力，这使它们具有对抗生物膜相关感染的巨大潜力。此外，它们可以通过同时抑制促炎细胞因子（TNF-α和IL-113）的表达和刺激趋化因子IL 8的表达来有效地调节大口黑鲈的抗感染免疫应答。动物攻毒试验证明，它们能显著提高大口黑鲈的攻毒存活率，使存活率由不足40%提高到60%以上，并能显著降低体内细菌数量。此外，与常规抗生素相比，它们显示出显著降低的诱导细菌耐药性的倾向。以上结果表明，这三种HDP在大口黑鲈养殖中作为新型抗感染药物具有很大的潜力。
Infectious diseases caused by drug-resistant bacteria are big challenges for aquaculture industry. Innovative antiinfective drugs and/or therapeutic strategies are urgently needed. Host defense peptides (HDPs) are naturally occurring peptides that are regarded as promising alternatives to conventional antibiotics. However, their application as antimicrobial agents in aquaculture has not been well studied. In the present study, three previously characterized HDPs (CATHPb1, Cm-CATH2, and Hc-CATH) were selected and their protective ability on largemouth bass (Micropterus salmoides) against bacterial infections was determined. The three HDPs exhibited potent and broad-spectrum antimicrobial activity against aquatic pathogenic bacteria, with MIC values ranging 2.34-18.75 mu g/ml. They rapidly kill bacteria within 30 min by inducing cytomembrane permeabilization and cell disruption. They also showed strong ability to inhibit the formation of bacterial biofilms and eradicate the preformed biofilms, which enable them with great potential to combat biofilm-related infections. Besides, they could effectively modulate the anti-infective immune responses of largemouth bass by simultaneously inhibiting the expression of pro-inflammatory cytokines (TNF-alpha and IL-113) and stimulating the expression of chemokine IL8. Animal challenge test proved that they could significantly enhance the survival rate of bacteria-challenged largemouth bass from less than 40% to over 60% and remarkably decrease the in vivo bacterial numbers. Moreover, compared to the conventional antibiotics, they showed dramatically lower propensity to induce bacterial resistance. All the results above imply the great potential of the three HDPs as novel anti-infective agents in largemouth bass culture.