Quantum yields of luminescent lanthanide chelates and far-red dyes measured by resonance energy transfer
Quantum yields of luminescent lanthanide chelates and far-red dyes measured by resonance energy transfer
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DOI：
10.1021/ja0031669
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发表时间：
2001-07-25
影响因子：
15
通讯作者：
Selvin, PR
中科院分区：
文献类型：
--
作者：
Xiao, M;Selvin, PR
发光稀土络合物具有不同寻常的光谱特性，这使它们成为传统有机荧光团的有价值的替代探针。然而，它们的量子产额、辐射衰减率和非辐射衰减率等基本参数一直很难或不可能测量。我们开发了一种基于共振能量传递的简单而稳健的方法来精确测量这些参数。此外，稀土络合物的激发/发射过程涉及几个步骤，我们能够量化每一步。这些包括有机天线的激发，从天线到稀土元素的能量转移，然后是稀土元素的发射。总体而言，这些参数表明稀土络合物可以是高效的长寿命发射体，使它们成为灵敏的检测试剂和共振能量转移的优秀施主。该方法也适用于用常规方法难以表征的红色发光染料的光物理表征。
Luminescent lanthanide chelates have unusual spectroscopic characteristics that make them valuable alternative probes to conventional organic fluorophores. However, fundamental parameters such as their quantum yield, and radiative and nonradiative decay rates have been difficult or impossible to measure. We have developed a simple and robust method based on resonance energy transfer to accurately measure these parameters. In addition, the excitation/emission process in lanthanide chelates involves several steps, and we are able to quantify each step. These include excitation of an organic antenna, transfer of energy from the antenna to lanthanide, and then Ianthanide emission. Overall, the parameters show that Ianthanide chelates can be efficient long-lived emitters, making them sensitive detection reagents and excellent donors in resonance energy transfer. The method is also shown to be applicable to photophysical characterization of red-emitting dyes, which are difficult to characterize by conventional means.