ENHANCED EXPRESSION OF THE C-MYC PROTOONCOGENE IN HIGH-GRADE HUMAN-PROSTATE CANCERS
ENHANCED EXPRESSION OF THE C-MYC PROTOONCOGENE IN HIGH-GRADE HUMAN-PROSTATE CANCERS
复制标题
DOI：
10.1002/pros.2990110405
复制
发表时间：
1987-01-01
期刊：
影响因子：
2.8
通讯作者：
OLSSON, CA
中科院分区：
文献类型：
--
作者：
BUTTYAN, R;SAWCZUK, IS;OLSSON, CA
我们检测了一系列29例良性和恶性前列腺组织的手术标本中Ha ‐ ras和c ‐ myc原癌基因的表达。从9例前列腺癌、19例良性前列腺肥大（BPH）和1例正常前列腺中提取多聚腺苷酸化mRNA，制备北方印迹。当北方印迹与探针forcHa‐ras杂交时，只有一个BPH标本显示出可观量的1.2 kb转录同源tocHa‐ras。在用c-myc重新探测这些印迹时，六种癌症显示出相当数量的与c-myc同源的2.4 kb转录物。其他三种癌症和所有良性组织显示很少或无法检测到2.4-kbc-myctranscript。在回顾性分析中，发现具有升高的dc-myctranscripts的癌症的Gleason评分为5分及以上（低分化肿瘤），而具有很少或noc-myctranscripts的癌症的Gleason评分均为4分及以下。最后，我们比较了我们检测从手术来源的前列腺肿瘤中提取的mRNA与从同一肿瘤中提取的mRNA的能力。电烙术降低了肿瘤mRNA中c-myc信号的强度和完整性。因此，我们仅使用手术来源的前列腺肿瘤可能是我们能够检测到高级别肿瘤中c-mycmRNA表达升高的原因。
We examined a series of 29 surgical specimens of benign and malignant human prostate tissue for the expression of both thecHa‐rasandc‐mycprotooncogenes. Northern blots were prepared using polyadenylated mRNA extracted from nine prostatic adenocarcinomas, 19 benign hypertrophied prostates (BPH) and one normal prostate. When the Northern blots were hybridized to a probe forcHa‐ras, only one specimen of BPH showed an appreciable amount of the 1.2‐kb transcript homologous tocHa‐ras. Upon reprobing these blots withc‐myc, six cancers showed a considerable amount of a 2.4‐kb transcript homologous toc‐myc. Three other cancers and all the benign tissue showed little or no detectable 2.4‐kbc‐myctranscript. On retrospective analysis, the cancers with elevatedc‐myctranscripts were found to have a Gleason score of 5 and above (poorly differentiated tumors), while the cancers with little or noc‐myctranscripts were all of Gleason score 4 and lower. Finally, we compared our ability to detectc‐myctranscripts in mRNA extracted from a surgically derived prostate tumor with mRNA extracted from the same tumor subject to a sham electrocautery procedure, as would occur during transurethral resection. The electrocautery procedure decreased both the intensity and the integrity of thec‐mycsignal in mRNA from the tumor. Thus, our exclusive use of surgically derived prostate tumors may be the reason we are able to detect an elevation in the expression ofc‐mycmRNA in high‐grade tumors.