Rapid and sensitive serum glucose determination using chemical labeling coupled with black phosphorus-assisted laser desorption/ionization time of-flight mass spectrometry
Rapid and sensitive serum glucose determination using chemical labeling coupled with black phosphorus-assisted laser desorption/ionization time of-flight mass spectrometry
复制标题
使用化学标记结合黑磷辅助激光解吸/电离飞行时间质谱法快速、灵敏地测定血清葡萄糖。
DOI：
10.1016/j.talanta.2017.08.055
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发表时间：
2018-01-01
期刊：
影响因子：
6.1
通讯作者：
Feng, Yu-Qi
中科院分区：
文献类型：
--
作者：
Wang, Qing;Yu, Lei;Feng, Yu-Qi
监测患者的血糖浓度是良好医疗诊断的关键组成部分。因此，制定一种准确、快速、灵敏的血糖监测策略至关重要。我们提出了一种结合2-（4-硼苯基）异喹啉-2-溴化ium化学标记和黑磷辅助激光解吸电离飞行时间质谱（CL-BP/ALDI-TOF MS）测定血清葡萄糖的方法。在BP/ALDI-TOF质谱分析之前，整个分析过程包括1分钟的蛋白质沉淀和3分钟的微波炉化学标记。分析可在5分钟内完成，具有高通量和极低的样品消耗。葡萄糖的线性关系良好，相关系数(R)为0.9986。检出限为11.5 fmol，定量限为37.5 fmol。日内精密度和日内精密度的相对标准偏差（rsd）均小于7.2%，相对加样回收率为87.1% ~ 108.1%，重现性和信度均较好。该方法还应用于各种血清样品的内源性葡萄糖分析，结果与临床实验室中使用己糖激酶法获得的结果一致。结果表明，本文提出的CL-BP/ALDI-TOF质谱方法可靠、快速、灵敏，可用于血清葡萄糖的定量分析。
Monitoring the concentration of blood glucose in patients is a key component of good medical diagnoses. Therefore, developing an accurate, rapid and sensitive strategy for monitoring blood glucose is of vital importance. We proposed a strategy for serum glucose determination combining 2-(4-boronobenzyl) isoquinolin-2-ium bromide chemical labeling with black phosphorus assisted laser desorption ionization-time of flight mass spectrometry (CL-BP/ALDI-TOF MS). The entire analytical process consisted of 1 min of protein precipitation and 3 min of chemical labeling in a microwave oven prior to the BP/ALDI-TOF MS analysis. The analysis can be completed in 5 min with high throughput and extremely low sample consumption. Good linearity for glucose was obtained with a correlation coefficient (R) of 0.9986. The limit of detection (LOD) and limit of quantification (LOQ) were 11.5 fmol and 37.5 fmol, respectively. Satisfied reproducibility and reliability were gained by evaluation of the intra- and inter-day precisions with relative standard deviations (RSDs) less than 7.2% and relative recoveries ranging from 87.1% to 108.1%, respectively. The proposed strategy was also applied for the analysis of endogenous glucose in various serum samples and the results were consistent with those obtained using the hexokinase method in a clinical laboratory. Considering the results, the proposed CL-BP/ALDI-TOF MS strategy has proven to be reliable, fast, and sensitive for quantitative analysis of serum glucose.