Patterns of protein oxidation in Arabidopsis seeds and during germination
Patterns of protein oxidation in Arabidopsis seeds and during germination
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DOI：
10.1104/pp.105.062778
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发表时间：
2005-06-01
期刊：
影响因子：
7.4
通讯作者：
Job, D
中科院分区：
文献类型：
--
作者：
Job, C;Rajjou, L;Job, D
已知在种子发育和萌发期间细胞活性氧水平增加，但蛋白质降解方面的后果特征不佳。在这项工作中，蛋白质羰基化，这是一个不可逆的氧化过程，导致功能丧失的修饰蛋白质，已被分析的蛋白质组学方法在拟南芥（拟南芥）种子萌发的第一阶段。在干燥成熟种子中，豆球蛋白型球蛋白（12 S十字花科植物蛋白）是主要的靶标。然而，酸性α-十字花科植物素亚基的羰基化程度比碱性（β）亚基高得多，这与β-亚基埋在十字花科植物素分子内而α-亚基更多地暴露于外部的模型一致.在吸胀过程中，各种羰基化蛋白质积累。这种氧化损伤是不均匀分布在种子蛋白质和目标特定的蛋白质糖酵解酶，线粒体ATP合酶，叶绿体核酮糖二磷酸羧化酶大链，醛糖还原酶，蛋氨酸合酶，翻译因子，和几个分子伴侣。虽然羰基化蛋白质的积累通常被认为是在各种模型系统中老化的背景下，这显然不是拟南芥种子的情况，因为它们以高速率发芽，并产生旺盛的植株。结果表明，所观察到的蛋白质羰基化模式的具体变化可能需要抵消和/或利用的生产引起的代谢活动的恢复发芽种子中的活性氧。
Increased cellular levels of reactive oxygen species are known to occur during seed development and germination, but the consequences in terms of protein degradation are poorly characterized. In this work, protein carbonylation, which is an irreversible oxidation process leading to a loss of function of the modified proteins, has been analyzed by a proteomic approach during the first stages of Arabidopsis ( Arabidopsis thaliana) seed germination. In the dry mature seeds, the legumin- type globulins ( 12S cruciferins) were the major targets. However, the acidic alpha- cruciferin subunits were carbonylated to a much higher extent than the basic ( beta) ones, consistent with a model in which the beta- subunits are buried within the cruciferin molecules and the a- subunits are more exposed to the outside. During imbibition, various carbonylated proteins accumulated. This oxidation damage was not evenly distributed among seed proteins and targeted specific proteins as glycolytic enzymes, mitochondrial ATP synthase, chloroplastic ribulose bisphosphate carboxylase large chain, aldose reductase, methionine synthase, translation factors, and several molecular chaperones. Although accumulation of carbonylated proteins is usually considered in the context of aging in a variety of model systems, this was clearly not the case for the Arabidopsis seeds since they germinated at a high rate and yielded vigorous plantlets. The results indicate that the observed specific changes in protein carbonylation patterns are probably required for counteracting and/ or utilizing the production of reactive oxygen species caused by recovery of metabolic activity in the germinating seeds.