Development of a multi-channel magnetic bead micro-probe assay for high-throughput detection of zearalenone in edible and medicinal Coix seed
Development of a multi-channel magnetic bead micro-probe assay for high-throughput detection of zearalenone in edible and medicinal Coix seed
复制标题
食药用薏苡仁中玉米赤霉烯酮多通道磁珠微探针高通量检测方法的开发
DOI：
10.1016/j.foodchem.2020.128977
复制
发表时间：
2021-01-23
期刊：
影响因子：
8.8
通讯作者：
Kong, Weijun
中科院分区：
文献类型：
--
作者：
Liu, Xiaofei;Liao, Xiaofang;Kong, Weijun
建立了基于间接竞争原理的多通道磁珠微探针法(MBPA)，用于食用和药用椰子中玉米赤霉烯酮(ZEA)的高通量检测。该策略引入磁珠作为载体，特异性一抗作为靶标捕获探针，二抗功能化羊抗鼠免疫球蛋白G标记的异硫氰酸荧光素作为荧光信号探针。通过检测样品中ZEA与其特异性抗体的竞争反应，以及快速磁分离和灵敏的荧光检测，该方法检测限低(2.03 ng/m L)，动态范围宽(2.03-440.67 ng/m L)，准确度高，平均回收率为96.39%，相对标准偏差为5.48%。36个样品在不到20min的一次操作中，只需要50亩L溶液和30个S样品，就可以实现同时分析，避免了大量的时间和有机溶剂的消耗。由于磁分离，省去了多次离心和净化步骤，避免了靶标的损失。不同的捕获和荧光探针可以随机结合到MBS的表面，使MBPA策略成为现场高通量监测食品安全和环境监测中各种痕量危害因素的一种有前景的工具。
A multi-channel magnetic bead micro-probes assay (MBPA) based on indirect competitive principle was developed for high-throughput detection of zearalenone (ZEA) in edible and medicinal Coix seed. This strategy introduced magnetic beads as the carriers, the specific primary antibodies as the capture probes for targets and the secondary antibodies functionalized goat anti-mouse immunoglobulin G labeled fluorescein isothiocyanate as the fluorescence signal probes. Through the competitive reaction of ZEA in Coix seed samples and that covalently coupled on the surface of MBs with their specific antibodies, as well as fast magnetic separation and sensitive fluorescence detection, the developed MBPA strategy allowed low limit of detection (2.03 ng/mL) with broad dynamic range (2.03-440.67 ng/mL), as well as excellent accuracy with the average recovery rate of 96.39% and relative standard deviation (RSD) of 5.48% for ZEA. 36 samples could realize simultaneous analysis in one operation within less than 20 min only needing 50 mu L of solution and 30 s of sampling, avoiding large consumption of time and organic solvents. Multiple centrifugation and cleanup steps were omitted because of magnetic separation, avoiding the loss of targets. Diverse capture and fluorescent probes can be randomly bound onto the surface of MBs, making the MBPA strategy a promising tool for on-site high-throughput monitoring of various trace hazard factors in food safety, and environmental monitoring.