HPLC-DAD-MS/MS ESI characterization of unusual highly glycosylated acylated flavonoids from cauliflower (Brassica oleracea L. var. botrytis) agroindustrial byproducts
HPLC-DAD-MS/MS ESI characterization of unusual highly glycosylated acylated flavonoids from cauliflower (Brassica oleracea L. var. botrytis) agroindustrial byproducts
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DOI：
10.1021/jf030077h
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发表时间：
2003-06-18
影响因子：
6.1
通讯作者：
Tomás-Barberán, FA
中科院分区：
文献类型：
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作者：
Llorach, R;Gil-Izquierdo, A;Tomás-Barberán, FA
在最近的一个项目中，我们研究了花椰菜的副产品，研究了将蔬菜处理和商业化的废物和残留物作为潜在生物活性提取物来源的可能性。共鉴定了28个化合物，其中22个化合物是由植物天然产生的。主要成分为山奈酚、3-二葡萄糖苷、7-葡萄糖苷及其与不同羟基肉桂酸的结合。此外，一些糖基化程度异常高的黄酮类化合物也是首次分离和初步鉴定。据我们所知，含有四种以上糖的类黄酮类化合物的特征以前还没有报道。初步鉴定为山奈酚-3-二葡萄糖苷-7-二葡萄糖苷、山奈酚-3-三糖苷-7-二葡萄糖苷、山奈酚-3-阿魏酰基-7-二葡萄糖苷、山奈酚-3-芥子酰基三葡糖苷-7-葡萄糖苷、山奈酚-3-二硫代葡萄糖苷-7-葡萄糖苷、山奈酚-3-芥子酰基三葡糖苷-7-二葡萄糖苷和山奈酚-3-二甘露糖苷-7-二葡萄糖苷。
In a recent program to investigate the possible use of vegetable handling and commercialization wastes and residues as a source of potentially bioactive extracts, we have studied cauliflower byproducts. A total of twenty-eight compounds were characterized by HPLC-DAD-MS, of these, twenty-two are produced naturally by the plant. The main compounds found were kaempferol 3-diglucoside7-glucoside and its combinations with different hydroxycinnamic acids. Moreover, some flavonoids with an unusual high grade of glycosylation have been isolated and tentatively identified for the first time. To our knowledge, the characterization of flavonoids with more than four sugars has not been previously reported. The new products isolated were tentatively identified as kaempferol 3-diglucoside-7-diglucoside, kaempferol 3-triglucoside-7-diglucoside, kaempferol 3-feruloyldiglucoside-7-diglucoside, kaempferol 3-sinapoyltriglucoside-7-glucoside, kaempferol 3-disinapoyltriglucoside-7-glucoside, kaempferol 3-sinapoyltriglucoside-7-diglucoside and kaempferol 3-disinapoyltriglucoside-7-diglucoside.