Reactive oxygen species and substance P in monocrotaline-induced pulmonary hypertension
Reactive oxygen species and substance P in monocrotaline-induced pulmonary hypertension
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DOI：
10.1006/taap.2000.9117
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发表时间：
2001-03-15
影响因子：
3.8
通讯作者：
Lai, YL
中科院分区：
文献类型：
--
作者：
Chen, MJ;Chiang, LY;Lai, YL
我们试图评价抗氧化剂1,3-二甲基-2-硫脲（DMTU）和六（磺基丁基）富勒烯（FC4S）是否通过降低Wister大鼠肺P物质（SP）来减轻单芥碱（MCT）诱导的肺动脉高压（PH）。体重297±8 g的大鼠63只，分为6组：对照组；未经中华人民共和国交通部;辣椒素+ MCT；MCT + dmtu-1；MCT + dmtu-2；MCT + FC4S。功能研究前3周，每只对照大鼠注射生理盐水，每只MCT大鼠注射60 mg/kg sc MCT。第三组大鼠接受辣椒素预处理后进行MCT治疗，在MCT后早期（DMTU-1）或后期（DMTU-2）注射DMTU 5天。对于最后一组，每只mct治疗的大鼠每天接受FC4S注射，直到功能研究开始。与对照组相比，MCT引起肺动脉压（Ppa）、右心室肥厚、肺动脉内侧厚度、肺SP水平和支气管肺泡灌洗中鲁米诺增强的化学发光计数显著增加。辣椒素和抗氧化剂均能显著减弱上述mct诱导的改变。sp诱导的急性Ppa升高在mct处理大鼠中被夸大。这些结果表明，氧自由基通过提高肺SP水平在mct诱导的PH中起重要作用。(C) 2001学术出版社。
We attempted to evaluate whether the antioxidants 1,3-dimethyl-2-thiourea (DMTU) and hexa(sulfobutyl)fullerenes (FC4S) attenuate monocrotaline (MCT)-induced pulmonary hypertension (PH) by lowering lung substance P (SP) in Wister rats. Sixty-three rats weighing 297 +/- 8 g were divided into six groups: control; MCT; capsaicin + MCT; MCT + DMTU-1; MCT + DMTU-2; and MCT + FC4S. Three weeks before the functional study, saline was injected into each control rat, whereas each MCT rat received 60 mg/kg sc MCT. Rats in the third group received capsaicin pretreatment followed by MCT, A S-day injection of DMTU was performed during the early (DMTU-1) or the late (DMTU-2) post-MCT period. For the last group, each MCT-treated rat received a daily FC4S injection until the commencement of the functional study. Compared to the control group, MCT caused significant increases in pulmonary arterial pressure (Ppa), right ventricular hypertropy, pulmonary arterial medial thickness, lung SP level, and luminol-enhanced chemiluminescence counts in bronchoalveolar lavage. Both capsaicin and antioxidants significantly attenuated the above MCT-induced alterations. SP-induced acute increase in Ppa was exaggerated in MCT-treated rats. These results suggest that oxygen radicals play an important role in MCT-induced PH via elevating lung SP level. (C) 2001 Academic Press.