Urinary cell-free nucleic acid IQGAP3: a new non-invasive diagnostic marker for bladder cancer.
Urinary cell-free nucleic acid IQGAP3: a new non-invasive diagnostic marker for bladder cancer.
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DOI：
10.18632/oncotarget.24436
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发表时间：
2018-03-06
期刊：
影响因子：
--
通讯作者：
Kim WJ
中科院分区：
文献类型：
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作者：
Kim WT;Kim YH;Jeong P;Seo SP;Kang HW;Kim YJ;Yun SJ;Lee SC;Moon SK;Choi YH;Lee GT;Kim IY;Kim WJ
人们越来越有兴趣开发新的膀胱癌（BC）非侵入性诊断工具，这些工具比膀胱镜检查和细胞学具有更好的敏感性和特异性。本研究探讨了尿游离核酸 (NA) 作为 BC 诊断标志物的价值。总共81名患者（74名BC和7名正常对照）用作组织组，212名患者（92名BC和120名正常对照）用作尿液组。然后检查组织 mRNA 和尿无细胞 NA 的表达。四个候选基因在组织微阵列中排名靠前。其中两种蛋白（IQGAP3 和 TOP2A）在 BC 患者的 BC 组织和尿液样本中的表达水平显着高于对照组样本。尿液样本中无细胞 NA 水平的二元逻辑回归分析显示，IQGAP3 与 BC 显着相关：PicoGreen 调整优势比 (OR)，3.434；置信区间 (CI)，2.999–4.180； P<0.001； RiboGreen 调整 OR，2.242； CI，1.793–2.840； P<0.001。对 IQGAP3 尿无细胞 NA 在肿瘤侵袭性和分级方面的进一步分析也产生了高 AUC，表明 IQGAP3 可以区分 BC 患者和血尿的非癌症患者。 BC患者尿中IQGAP3无细胞NA水平显着高于正常对照或血尿患者。高水平的 IQGAP3 尿液无细胞 NA 也反映了 BC 组织中的高表达。因此，IQGAP3 尿液无细胞 NA 可能是 BC 的补充诊断生物标志物。
There is growing interest in developing new non-invasive diagnostic tools for bladder cancer (BC) that have better sensitivity and specificity than cystoscopy and cytology. This study examined the value of urinary cell-free nucleic acid (NA) as a diagnostic marker for BC. A total of 81 patients (74 BC and 7 normal controls) were used for a tissue set, and 212 patients (92 BC and 120 normal controls) were used as a urine set. Expression of tissue mRNA and urinary cell-free NAs was then examined. Four candidate genes were top-ranked in the tissue microarray. Expression levels of two of these (IQGAP3 and TOP2A) in BC tissue and urine samples from BC patients were significantly higher than those in samples from the control groups. Binary logistic regression analysis of cell-free NA levels in urine samples revealed that IQGAP3 was significantly associated with BC: PicoGreen-adjusted odds ratio (OR), 3.434; confidence interval (CI), 2.999–4.180; P<0.001; RiboGreen-adjusted OR, 2.242; CI, 1.793–2.840; P<0.001. Further analysis of IQGAP3 urinary cell-free NAs with respect to tumor invasiveness and grade also yielded a high AUC, suggesting that IQGAP3 can discriminate between BC patients and non-cancer patients with hematuria. Levels of IQGAP3 urinary cell-free NA in BC patients were significantly higher than those in normal controls or patients with hematuria. High levels of IQGAP3 urinary cell-free NA also reflected high expression in BC tissues. Therefore, IQGAP3 urinary cell-free NA may be a complementary diagnostic biomarker for BC.